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BRF1 antibody

Anti-BRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55860
Anti-BRF1 antibody used in Western Blot (WB). GTX55860
Anti-BRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55860

Cat. No. GTX55860

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all BRF1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

74 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of BRF1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BRF1 RNA polymerase III transcription initiation factor subunit , BRF , BRF-1 , CFDS , GTF3B , HEL-S-76p , TAF3B2 , TAF3C , TAFIII90 , TF3B90 , TFIIIB90 , hBRF

Cellular Localization

Nucleus

Background

This gene encodes one of the three subunits of the RNA polymerase III transcription factor complex. This complex plays a central role in transcription initiation by RNA polymerase III on genes encoding tRNA, 5S rRNA, and other small structural RNAs. The gene product belongs to the TF2B family. Several alternatively spliced variants encoding different isoforms, that function at different promoters transcribed by RNA polymerase III, have been identified. [provided by RefSeq, Jun 2011]

Database

DATA IMAGES

Anti-BRF1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55860

GTX55860 ICC/IF Image

ICC/IF analysis of formalin-fixed NIH3T3 cells using GTX55860 BRF1 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-BRF1 antibody used in Western Blot (WB). GTX55860

GTX55860 WB Image

WB analysis of Jurkat (A), NIH3T3 (B) whole cell lysates using GTX55860 BRF1 antibody.

Anti-BRF1 antibody used in IHC (Paraffin sections) (IHC-P). GTX55860

GTX55860 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX55860 BRF1 antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399