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BRG1 antibody [6D7]

Anti-BRG1 antibody [6D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66842
Anti-BRG1 antibody [6D7] used in Immunoprecipitation (IP). GTX66842
Anti-BRG1 antibody [6D7] used in Western Blot (WB). GTX66842

Cat. No. GTX66842

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D7

Isotype

 IgG

Application

 WB, ICC/IF, IP

Reactivity

 Human, Mouse
Package
100 μl ($459)
See all BRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:50-1:200
IP 1:50-1:200
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.2% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human SMARCA4 protein fragments expressed in E.coli.

Purification

Purified by antigen-affinity chromatography

Purity

> 95% (by SDS-PAGE)

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-BRG1 antibody [6D7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX66842

GTX66842 ICC/IF Image

ICC/IF analysis of 4% PFA-fixed HeLa cells using GTX66842 BRG1 antibody [6D7].
Blue : DAPI
Dilution : 1:50

Anti-BRG1 antibody [6D7] used in Immunoprecipitation (IP). GTX66842

GTX66842 IP Image

IP analysis of K562 cell lysates using GTX66842 BRG1 antibody [6D7].

Anti-BRG1 antibody [6D7] used in Western Blot (WB). GTX66842

GTX66842 WB Image

WB analysis of HeLa NE, F9, and K562 cell lysates using GTX66842 BRG1 antibody [6D7].
Dilution : 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 459