APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
K562 , Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat2 , 293T
Predict Reactivity
Bovine, Chicken(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
2.44 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human BRG1. The exact sequence is proprietary.
Purification
Affinity purified by Protein G.
Conjugation
Unconjugated
RRID
AB_2888340
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b
Cellular Localization
Nucleus
Background
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/templates/default/js/lightbox/images/loading.gif "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
|
GTX633391 WB Image
Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
|
|
|
GTX633391 IHC-P Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human endometrial carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human endometrial carcinoma.
BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633391 IHC-P Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus in human oral carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human oral carcinoma.
BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
|
|
|
GTX633391 IHC-Fr Image
BRG1 antibody [GT2712] detects BRG1 protein by immunohistochemical analysis.Sample: Frozen-sectioned rat E13.5 brain.Green: BRG1 stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:250.Red: SOX2, stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
|
|
|
GTX633391 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
|
|
GTX633391 WB Image
Whole cell extract (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
|
|
GTX633391 ICC/IF Image
BRG1 antibody [GT2712] detects BRG1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRG1 protein stained by BRG1 antibody [GT2712] (GTX633391) diluted at 1:100.
Red: phalloidin, a cytoskeleton marker, diluted at 1:50.
Blue: Hoechst 33342 staining.
|
|
|
GTX633391 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody [GT2712] (GTX633391) diluted at 1:1000.
|
REFERENCE
There are currently no references for BRG1 antibody [GT2712] (GTX633391). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BRG1 antibody [GT2712] (GTX633391). Be the first to share your experience with this product.
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20180216_WB_KO_watermark_w_23061202_386.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160615_IHC-P_2_w_23061202_163.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160615_IHC-P_w_23061202_117.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20171106_IHC-Fr_R_w_23061202_767.webp "Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20161222_WB_M_w_23061202_107.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160505_WB_w_23061202_653.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20160706_IFA_w_23061202_763.webp "Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/small/GTX633391/GTX633391_42478_20161222_WB_R_w_23061202_650.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20180216_WB_KO_watermark_w_23061202_386.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_2_w_23061202_163.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160615_IHC-P_w_23061202_117.webp "Anti-BRG1 antibody [GT2712] used in IHC (Paraffin sections) (IHC-P). GTX633391")
![Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20171106_IHC-Fr_R_w_23061202_767.webp "Anti-BRG1 antibody [GT2712] used in IHC (Frozen sections) (IHC-Fr). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_M_w_23061202_107.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160505_WB_w_23061202_653.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20160706_IFA_w_23061202_763.webp "Anti-BRG1 antibody [GT2712] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX633391")
![Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391](/upload/website/prouct_img/normal/GTX633391/GTX633391_42478_20161222_WB_R_w_23061202_650.webp "Anti-BRG1 antibody [GT2712] used in Western Blot (WB). GTX633391")