BRG1 antibody

Cat. No. GTX00727

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IP
Reactivity	Human, Mouse
Package	100 μg ($399)

See all BRG1 products

Datasheet
Document

View product citations for antibody GTX00727 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:1000
ICC/IF	1:1000
IP	Assay dependent

Not tested in other applications.

Calculated MW

185 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% Glycerol

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal 50 amino acids of human BRG1, C-KLGRKEKAQDRLK GGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED, conjugated with KLH

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Gene ID: 6597 SMARCA4
UniProt: P51532 SMARCA4

Research Area

DATA IMAGES

GTX00727 IP Image

IP analysis of HeLa nuclear extracts using GTX00727 BRG1 antibody.
Lane 1 : Input nuclear extracts
Lane 2 : Control IP with non-immune IgG
Lane 3 : Immunoprecipitation with anti-Brg1 antibody

GTX00727 WB Image

WB analysis of various samples using GTX00727 BRG1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Dilution : 1:1000

GTX00727 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00727 BRG1 antibody.
Dilution : 1:1000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf

Package

List Price ($)

100 μg

$ 399

Qty