ICC/IF analysis of HeLa cells using GTX00727 BRG1 antibody.
Dilution : 1:1000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

WB analysis of various samples using GTX00727 BRG1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Dilution : 1:1000

IP analysis of HeLa nuclear extracts using GTX00727 BRG1 antibody.
Lane 1 : Input nuclear extracts
Lane 2 : Control IP with non-immune IgG
Lane 3 : Immunoprecipitation with anti-Brg1 antibody