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BRG1 antibody

Anti-BRG1 antibody used in Immunoprecipitation (IP). GTX00727
Anti-BRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00727
Anti-BRG1 antibody used in Western Blot (WB). GTX00727

Cat No. GTX00727

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IP

Reactivity

Human, Mouse
Package
100 μg ($349)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000
ICC/IF 1:1000
IP Assay dependent
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Filter-sterilized PBS, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

C-terminal 50 amino acids of human BRG1, C-KLGRKEKAQDRLK GGRRRPSRGSRAKPVVSDDDSEEEQEEDRSGSGSEED, conjugated with KLH

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-BRG1 antibody used in Immunoprecipitation (IP). GTX00727

GTX00727 IP Image

IP analysis of HeLa nuclear extracts using GTX00727 BRG1 antibody.
Lane 1 : Input nuclear extracts
Lane 2 : Control IP with non-immune IgG
Lane 3 : Immunoprecipitation with anti-Brg1 antibody

Anti-BRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX00727

GTX00727 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX00727 BRG1 antibody.
Dilution : 1:1000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min

Anti-BRG1 antibody used in Western Blot (WB). GTX00727

GTX00727 WB Image

WB analysis of various samples using GTX00727 BRG1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : NIH3T3 whole cell lysate
Dilution : 1:1000

REFERENCE

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REVIEW

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Package List Price ($)
$ 349