*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
293T , A431 , HeLa , HepG2
Mouse, Rat, Bovine, Chicken(>80% identity)
1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
0.82 mg/ml (Please refer to the vial label for the specific concentration.)
Recombinant protein encompassing a sequence within the N-terminus region of Human BRG1. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4,BAF190,BAF190A,BRG1,CSS4,MRD16,RTPS2,SNF2,SNF2L4,SNF2LB,SWI2,hSNF2b
The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]
GTX132121 WB Image
Wild-type (WT) and BRG1 knockout (KO) 293T cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody (GTX132121) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
GTX132121 ICC/IF Image
BRG1 antibody detects BRG1 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BRG1 protein stained by BRG1 antibody (GTX132121) diluted at 1:1000.
Red: Phalloidin, a cytoskeleton marker, diluted at 1:100.
GTX132121 WB Image
Various whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BRG1 antibody (GTX132121) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.