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BRG1 antibody

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917
Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917
Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917
Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917
Anti-BRG1 antibody used in Western Blot (WB). GTX31917

Cat No. GTX31917

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, ELISA

Reactivity

Human, Mouse, Rat
Package
100 μg ($369)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1 - 2 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

Positive Control

SK-N-SH Cell Lysate(GTX23956)

PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BRG1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human BRG1.The immunogen is located within amino acids 1420 - 1470 of BRG1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917

GTX31917 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX31917 BRG1 antibody.
Working concentration : 2 μg/ml

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917

GTX31917 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31917 BRG1 antibody.
Working concentration : 5 μg/ml

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917

GTX31917 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX31917 BRG1 antibody.
Working concentration : 20 μg/ml

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31917

GTX31917 IHC-P Image

IHC-P analysis of rat brain tissue using GTX31917 BRG1 antibody.
Working concentration : 5 μg/ml

Anti-BRG1 antibody used in Western Blot (WB). GTX31917

GTX31917 WB Image

WB analysis of SK-N-SH cell lysate using GTX31917 BRG1 antibody.
Working concentration : 1 μg/ml

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf