GeneTex

  • Country / Location Selection

United States (US)

BRG1 antibody

KO/KD
Anti-BRG1 antibody used in Western Blot (WB). GTX35204
Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35204
Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35204
Anti-BRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35204
Anti-BRG1 antibody used in Western Blot (WB). GTX35204

Cat. No. GTX35204

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399)
See all BRG1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:50
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

185 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide of human SMARCA4

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4 , BAF190 , BAF190A , BRG1 , CSS4 , MRD16 , RTPS2 , SNF2 , SNF2L4 , SNF2LB , SWI2 , hSNF2b

Cellular Localization

Nucleus

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]

Database

Research Area

DATA IMAGES

Anti-BRG1 antibody used in Western Blot (WB). GTX35204

GTX35204 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX35204 BRG1 antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35204

GTX35204 IHC-P Image

IHC-P analysis of human colon tissue using GTX35204 BRG1 antibody.
Dilution : 1:100

Anti-BRG1 antibody used in IHC (Paraffin sections) (IHC-P). GTX35204

GTX35204 IHC-P Image

IHC-P analysis of human colon carcinoma tissue using GTX35204 BRG1 antibody.
Dilution : 1:100

Anti-BRG1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX35204

GTX35204 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX35204 BRG1 antibody.

Anti-BRG1 antibody used in Western Blot (WB). GTX35204

GTX35204 WB Image

WB analysis of various sample lysates using GTX35204 BRG1 antibody.
Dilution : 1:500
Loading : 25μg per lane

REFERENCE

Application Reference

Panditharatna E et al. Cancer Discov 2022; 12 (12) : 2880-2905 BAF Complex Maintains Glioma Stem Cells in Pediatric H3K27M Glioma.
Submit a Reference

REVIEW

There are currently no reviews for BRG1 antibody (GTX35204). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399