GeneTex
United States (US)

BRG1 antibody

KO/KD
GTX35204_20180822_WB_1_18121410_416.jpg
GTX35204_20180822_IHC-P_18121410_822.jpg
GTX35204_20180822_IHC-P_1_18121410_208.jpg
GTX35204_20180822_WB_18121410_168.jpg

Cat No. GTX35204

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

185 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 50% glycerol, 0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

A synthetic peptide of human SMARCA4

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Swi/Snf Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily A, Member 4,Baf190,Baf190A,Brg1,Css4,Mrd16,Rtps2,Snf2,Snf2L4,Snf2Lb,Swi2,Hsnf2B,Smarca4

Background

The protein encoded by this gene is a member of the SWI/SNF family of proteins and is similar to the brahma protein of Drosophila. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The encoded protein is part of the large ATP-dependent chromatin remodeling complex SNF/SWI, which is required for transcriptional activation of genes normally repressed by chromatin. In addition, this protein can bind BRCA1, as well as regulate the expression of the tumorigenic protein CD44. Mutations in this gene cause rhabdoid tumor predisposition syndrome type 2. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2012]
DATA IMAGES
GTX35204_20180822_WB_1_18121410_416.jpg

GTX35204 WB Image

KO/KD

WB analysis of wild type and BRG1 knockout cell lysate using GTX35204 BRG1 antibody.
Dilution : 1:500
Loading : 25μg per lane

GTX35204_20180822_IHC-P_18121410_822.jpg

GTX35204 IHC-P Image

KO/KD

IHC-P analysis of human colon tissue using GTX35204 BRG1 antibody.
Dilution : 1:100

GTX35204_20180822_IHC-P_1_18121410_208.jpg

GTX35204 IHC-P Image

KO/KD

IHC-P analysis of human colon carcinoma tissue using GTX35204 BRG1 antibody.
Dilution : 1:100

GTX35204_20180822_WB_18121410_168.jpg

GTX35204 WB Image

KO/KD

WB analysis of various samples using GTX35204 BRG1 antibody.
Dilution : 1:500
Loading : 25μg per lane

Package List Price ($)
$ 329