GeneTex
United States (US)

BRRN1 antibody [N1N2], N-term

GTX115699_40499_IFA_18121410_175.jpg
GTX115699_40499_WB_18121410_391.jpg
GTX115699_40499_IHC_18121410_631.jpg

Cat No. GTX115699

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

Positive Control

HeLa

Predict Reactivity

Mouse, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BRRN1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Non-Smc Condensin I Complex Subunit H,Brrn1,Cap-H,Caph,Mcph23,Ncaph

Cellular Localization

Nucleus , Cytoplasm

Background

This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX115699_40499_IFA_18121410_175.jpg

GTX115699 ICC/IF Image

BRRN1 antibody [N1N2], N-term detects BRRN1 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: BRRN1 protein stained by BRRN1 antibody [N1N2], N-term (GTX115699) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115699_40499_WB_18121410_391.jpg

GTX115699 WB Image

Sample (30 ug of whole cell lysate)
A: HeLa
7.5% SDS PAGE
GTX115699 diluted at 1:500

GTX115699_40499_IHC_18121410_631.jpg

GTX115699 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using BRRN1(GTX115699) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169