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BSA antibody [5F9]

Anti-BSA antibody [5F9] used in ELISA (ELISA). GTX29092
Anti-BSA antibody [5F9] used in Western Blot (WB). GTX29092

Cat. No. GTX29092

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 5F9

Isotype

 IgG1

Application

 WB, IP, ELISA, Functional Assay, IHC

Reactivity

 Bovine
Package
100 μg ($399)
See all BSA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000-1:10000
IP 1:100
ELISA 1:120000
Functional Assay Assay dependent
IHC 1:1000-1:5000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Product Note

The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer. No cross reactivity is detected against other serum proteins by immunoblot. No reactivity is detected by ELISA against human serum albumin or ovalbumin.

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Bovine serum albumin

Purification

Protein A purified
From tissue cultue supernatant

Conjugation

Unconjugated

RRID

AB_1949461

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

albumin

Cellular Localization

Secreted

Background

Serum albumin, a globular unglycosylated protein of molecular weight 65 kDa, is the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.

Database

Research Area

DATA IMAGES

Anti-BSA antibody [5F9] used in ELISA (ELISA). GTX29092

GTX29092 ELISA Image

ELISA analysis of BSA using serially diluted GTX29092 BSA antibody [5F9].
Coating : 10 μg

Anti-BSA antibody [5F9] used in Western Blot (WB). GTX29092

GTX29092 WB Image

WB analysis of bovine BSA protein using GTX29092 BSA antibody [5F9].
Loading : 50 ng
Dilution : 1:1000

REFERENCE

Application Reference

Chuang CH et al. Biosens Bioelectron 2013; 48 : 158-64 Enhancing of intensity of fluorescence by DEP manipulations of polyaniline-coated Al2O3 nanoparticles for immunosensing.
Application : Functional Assay
Submit a Reference

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399