GeneTex
United States (US)

BSA antibody [5F9]

Cat No. GTX29092

Host Mouse
Clonality Monoclonal
Clone Name 5F9
Isotype IgG1
Application WB, IP, ELISA, Functional Assay, IHC
Reactivity Bovine
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:2,000 - 1:10,000
IP 1:100
ELISA 1:120,000
Functional Assay Assay dependent
IHC 1:1,000 - 1:5,000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Specificity/Sensitivity

The antibody recognizes a 66 kDa band corresponding to bovine serum albumin monomer.  No cross reactivity is detected against other serum proteins by immunoblot.  No reactivity is detected by ELISA against human serum albumin or ovalbumin.
PROPERTIES

Form

Liquid

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 and 0.01% (w/v) Sodium Azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Bovine

Immunogen

Anti-Bovine Serum Albumin antibody was produced by repeated immunizations of balb/c mice with bovine serum albumin.

Purification

Protein A purified

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Albumin,Alb

Background

Serum albumin, a globular unglycosylated protein of molecular weight 65 kDa, is the main protein of plasma, has a good binding capacity for water, Ca2+, Na+, K+, fatty acids, hormones, bilirubin and drugs. Its main function is the regulation of the colloidal osmotic pressure of blood. Albumin is synthesized in the liver as preproalbumin, which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to give the secreted albumin.

Database

Research Area

REFERENCE
Package List Price ($)
$ 289