GeneTex
United States (US)

BSEP antibody [N3C1], Internal

GTX102608_39742_IHC_19011506_974.jpg
GTX102608_WB_19011506_155.jpg
GTX102608_39742_IFA_19011506_730.jpg

Cat No. GTX102608

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

146 kDa. ( Note )

Positive Control

Jurkat

Predict Reactivity

Mouse, Rat, Rabbit, Bovine, Cat, Dog, Guinea pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

9.01mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BSEP. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

ABC16 antibody, BRIC2 antibody, BSEP antibody, PFIC-2 antibody, PFIC2 antibody, PGY4 antibody, SPGP antibody, ABCB11 antibody, ATP-binding cassette sub-family B member 11 antibody, sister p-glycoprotein antibody, bile salt export pump antibody, progressive familial intrahepatic cholestasis 2 antibody, ABC member 16, MDR/TAP subfamily antibody, ATP-binding cassette, sub-family B (MDR/TAP), member 11 antibody

Cellular Localization

Membrane

Background

The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq]

Database

DATA IMAGES
GTX102608_39742_IHC_19011506_974.jpg

GTX102608 IHC-P Image

Immunohistochemical analysis of paraffin-embedded AGS xenograft, using BSEP(GTX102608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102608_WB_19011506_155.jpg

GTX102608 WB Image

Sample (30 µg of whole cell lysate)
A: JurKat
5% SDS PAGE
GTX102608 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX102608_39742_IFA_19011506_730.jpg

GTX102608 ICC/IF Image

BSEP antibody [N3C1], Internal detects ABCB11 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ABCB11 protein stained by BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:500.
Blue: Hoechst 33343 staining.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169