APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Jurkat
Predict Reactivity
Rat, Rabbit, Bovine, Cat, Dog, Guinea pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.17 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the Intracellular domain of human BSEP. The exact sequence is proprietary.
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
RRID
AB_2036139
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
ATP binding cassette subfamily B member 11 , ABC16 , BRIC2 , BSEP , PFIC-2 , PFIC2 , PGY4 , SPGP
Cellular Localization
Membrane
Background
The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance. The protein encoded by this gene is the major canalicular bile salt export pump in man. Mutations in this gene cause a form of progressive familial intrahepatic cholestases which are a group of inherited disorders with severe cholestatic liver disease from early infancy. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608](/templates/default/js/lightbox/images/loading.gif "Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608")
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GTX102608 WB Image
Sample (30 μg of whole cell lysate)
A: JurKat
5% SDS PAGE
GTX102608 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX102608 IHC-P Image
BSEP antibody [N3C1], Internal detects BSEP protein at cell membrane by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.BSEP stained by BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:500.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX102608 ICC/IF Image
BSEP antibody [N3C1], Internal detects ABCB11 protein at membrane by immunofluorescent analysis.
Sample: A431 cells were fixed in ice-cold MeOH for 5 min.
Green: ABCB11 protein stained by BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:500.
Blue: Hoechst 33343 staining.
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GTX102608 WB Image
Various tissue extracts (5 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BSEP antibody [N3C1], Internal (GTX102608) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
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GTX102608 IHC-P Image
Immunohistochemical analysis of paraffin-embedded AGS xenograft, using BSEP(GTX102608) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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REFERENCE
There are currently no references for BSEP antibody [N3C1], Internal (GTX102608). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BSEP antibody [N3C1], Internal (GTX102608). Be the first to share your experience with this product.
![Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608](/upload/website/prouct_img/small/GTX102608/GTX102608_WB_w_23060119_348.webp "Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608](/upload/website/prouct_img/small/GTX102608/GTX102608_44636_20220422_IHC-P_M_w_23060119_459.webp "Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102608](/upload/website/prouct_img/small/GTX102608/GTX102608_39742_IFA_w_23060119_742.webp "Anti-BSEP antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608](/upload/website/prouct_img/small/GTX102608/GTX102608_44517_20220107_WB_tissue_w_23060119_550.webp "Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608](/upload/website/prouct_img/small/GTX102608/GTX102608_39742_IHC_w_23060119_696.webp "Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608](/upload/website/prouct_img/normal/GTX102608/GTX102608_WB_w_23060119_348.webp "Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608](/upload/website/prouct_img/normal/GTX102608/GTX102608_44636_20220422_IHC-P_M_w_23060119_459.webp "Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102608](/upload/website/prouct_img/normal/GTX102608/GTX102608_39742_IFA_w_23060119_742.webp "Anti-BSEP antibody [N3C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608](/upload/website/prouct_img/normal/GTX102608/GTX102608_44517_20220107_WB_tissue_w_23060119_550.webp "Anti-BSEP antibody [N3C1], Internal used in Western Blot (WB). GTX102608")
![Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608](/upload/website/prouct_img/normal/GTX102608/GTX102608_39742_IHC_w_23060119_696.webp "Anti-BSEP antibody [N3C1], Internal used in IHC (Paraffin sections) (IHC-P). GTX102608")