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BTF antibody

Orthogonal
Anti-BTF antibody used in IHC (Paraffin sections) (IHC-P). GTX130772
Anti-BTF antibody used in IHC (Paraffin sections) (IHC-P). GTX130772
Anti-BTF antibody used in Western Blot (WB). GTX130772
Anti-BTF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130772

Cat. No. GTX130772

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BTF products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

106 kDa. ( Note )

Positive Control

K562 nuclear extract

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.08 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BTF . The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886343

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated transcription factor 1 , BTF , bK211L9.1

Cellular Localization

Cytoplasm , Nucleus

Background

This gene encodes a transcriptional repressor that interacts with several members of the BCL2 family of proteins. Overexpression of this protein induces apoptosis, which can be suppressed by co-expression of BCL2 proteins. The protein localizes to dot-like structures throughout the nucleus, and redistributes to a zone near the nuclear envelope in cells undergoing apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BTF antibody used in IHC (Paraffin sections) (IHC-P). GTX130772

GTX130772 IHC-P Image

BTF antibody detects BTF protein at nucleus in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
BTF antibody (GTX130772) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BTF antibody used in IHC (Paraffin sections) (IHC-P). GTX130772

GTX130772 IHC-P Image

BTF antibody detects BTF protein at nucleus in mouse cervix by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cervix.
BTF antibody (GTX130772) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BTF antibody used in Western Blot (WB). GTX130772

GTX130772 WB Image

Orthogonal

K562 whole cell and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BTF antibody (GTX130772) diluted at 1:1000.

Anti-BTF antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130772

GTX130772 ICC/IF Image

BTF antibody detects BTF protein at nucleus by immunofluorescent analysis.
Sample: MCF7 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: BTF protein stained by BTF antibody (GTX130772) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169