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BTK antibody [10E10]

Overexpression
Anti-BTK antibody [10E10] used in IHC (Paraffin sections) (IHC-P). GTX84785
Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785
Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785
Anti-BTK antibody [10E10] used in Flow cytometry (FACS). GTX84785
Anti-BTK antibody [10E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84785
Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785
Anti-BTK antibody [10E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84785

Cat. No. GTX84785

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 10E10

Isotype

 IgG2a

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Mouse
Package
100 μl ($519)
See all BTK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS Assay dependent
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA

Cellular Localization

Cytoplasm,Nucleus

Background

Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.

Database

Research Area

DATA IMAGES

Anti-BTK antibody [10E10] used in IHC (Paraffin sections) (IHC-P). GTX84785

GTX84785 IHC-P Image

IHC-P analysis of human liver tissue using GTX84785 BTK antibody [10E10].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785

GTX84785 WB Image

WB analysis of various samples using GTX84785 BTK antibody [10E10].
Loading : 10 ug per lane
Dilution : 1:200

Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785

GTX84785 WB Image

Overexpression

WB analysis of HEK293T cells transfected with BTK plasmid (Right) or empty vector (Left) for 48 hrs using GTX84785 BTK antibody [10E10].
Loading : 5 ug per lane

Anti-BTK antibody [10E10] used in Flow cytometry (FACS). GTX84785

GTX84785 FACS Image

FACS analysis of monocytes from non-XLA or XLA patients using GTX84785 BTK antibody [10E10].

Anti-BTK antibody [10E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84785

GTX84785 ICC/IF Image

ICC/IF analysis of HT29 cells using GTX84785 BTK antibody [10E10].

Anti-BTK antibody [10E10] used in Western Blot (WB). GTX84785

GTX84785 WB Image

WB analysis of various cell lines using GTX84785 BTK antibody [10E10].
Loading : 10 ug per lane
Dilution : 1:200

Anti-BTK antibody [10E10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84785

GTX84785 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BTK plasmid using GTX84785 BTK antibody [10E10].

REFERENCE

Application Reference

Kwak SC et al. Nutrients 2020; 12 (10) Grape Seed Proanthocyanidin Extract Prevents Bone Loss via Regulation of Osteoclast Differentiation, Apoptosis, and Proliferation.
Application : WB
Reactivity : Mouse
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519