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BTK antibody [12H4]

Anti-BTK antibody [12H4] used in Western Blot (WB). GTX84792
Anti-BTK antibody [12H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84792
Anti-BTK antibody [12H4] used in IHC (Paraffin sections) (IHC-P). GTX84792
Anti-BTK antibody [12H4] used in Flow cytometry (FACS). GTX84792
Anti-BTK antibody [12H4] used in Flow cytometry (FACS). GTX84792

Cat. No. GTX84792

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 12H4

Isotype

 IgG3

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human
Package
100 μl ($519)
See all BTK products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:100
IHC-P 1:50
FACS 1:100
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human recombinant protein of human BTK (NP_000052) produced in HEK293T cell.

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA

Cellular Localization

Cytoplasm,Nucleus

Background

Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.

Database

Research Area

DATA IMAGES

Anti-BTK antibody [12H4] used in Western Blot (WB). GTX84792

GTX84792 WB Image

WB analysis of HEK293T cells transfected with BTK plasmid (Right) or empty vector (Left) for 48 hrs using GTX84792 BTK antibody [12H4].
Loading : 5 ug per lane

Anti-BTK antibody [12H4] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84792

GTX84792 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BTK plasmid using GTX84792 BTK antibody [12H4].

Anti-BTK antibody [12H4] used in IHC (Paraffin sections) (IHC-P). GTX84792

GTX84792 IHC-P Image

IHC-P analysis of liver tissue using GTX84792 BTK antibody [12H4].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-BTK antibody [12H4] used in Flow cytometry (FACS). GTX84792

GTX84792 FACS Image

FACS analysis of HEK293T cells transfected with either BTK plasmid(Red) or empty vector control plasmid(Blue) using GTX84792 BTK antibody [12H4].

Anti-BTK antibody [12H4] used in Flow cytometry (FACS). GTX84792

GTX84792 FACS Image

FACS analysis of Jurkat cells using GTX84792 BTK antibody [12H4].
Red : Primary antibody
Blue : Negative control antibody

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519