GeneTex
United States (US)

BTK antibody [C1C3]

GTX101346_39911_IHC_M_19011506_400.jpg
GTX101346_WB_19011506_860.jpg
GTX101346_39911_IHC_19011506_279.jpg

Cat No. GTX101346

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Molt-4

Predict Reactivity

Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.62mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human BTK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bruton Tyrosine Kinase,Agmx1,At,Atk,Bpk,Imd1,Psctk1,Xla,Btk

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus

Background

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101346_39911_IHC_M_19011506_400.jpg

GTX101346 IHC-P Image

BTK antibody [C1C3] detects BTK protein at cytosol on mouse spleen by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
BTK antibody [C1C3] (GTX101346) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101346_WB_19011506_860.jpg

GTX101346 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
7.5% SDS PAGE
GTX101346 diluted at 1:500

GTX101346_39911_IHC_19011506_279.jpg

GTX101346 IHC-P Image

Immunohistochemical analysis of paraffin-embedded SAS xenograft, using BTK(GTX101346) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169