APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| Assay dependent |
Not tested in other applications.
Calculated MW
Predict Reactivity
Rat, Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
No preservative
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein of human BTK
Purification
Affinity purified by Protein A.
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA
Cellular Localization
Cytoplasm,Nucleus
Background
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2013]
Database
Research Area
DATA IMAGES
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/templates/default/js/lightbox/images/loading.gif "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
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GTX637455 WB Image
Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637455 ICC/IF Image
BTK antibody [HL1803] detects BTK protein by immunofluorescent analysis.Sample: K562 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: BTK stained by BTK antibody [HL1803] (GTX637455) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX637455 WB Image
Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK antibody [HL1803] (GTX637455) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for BTK antibody [HL1803] (GTX637455). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for BTK antibody [HL1803] (GTX637455). Be the first to share your experience with this product.
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20230616_WB_shRNA_watermark_23062019_226.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20221230_ICC_IF_23010219_591.webp "Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20221111_WB_22111518_162.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_T-44809_20221021_WB_M_22102723_676.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/small/GTX637455/GTX637455_44858_20230421_WB_TPM_watermark_23042500_514.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20230616_WB_shRNA_watermark_23062019_226.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20221230_ICC_IF_23010219_591.webp "Anti-BTK antibody [HL1803] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20221111_WB_22111518_162.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_T-44809_20221021_WB_M_22102723_676.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")
![Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455](/upload/website/prouct_img/normal/GTX637455/GTX637455_44858_20230421_WB_TPM_watermark_23042500_514.webp "Anti-BTK antibody [HL1803] used in Western Blot (WB). GTX637455")