APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:1000-1:10000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
Raji (1 mM pervanadate treatment for 30 min)
Predict Reactivity
Bovine, Chicken, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 1% BSA, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide surrounding phospho Tyr223 of human BTK. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886249
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA
Cellular Localization
Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus
Background
The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse spleen.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130377 WB Image
Untreated (–) and treated (+) Raji whole cell extracts (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:6000.
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded mouse thymus gland.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX130377 IHC-P Image
BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.Sample: Paraffin-embedded rat lymph node.BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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