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BTK (phospho Tyr223) antibody

Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377
Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377
Anti-BTK (phospho Tyr223) antibody used in Western Blot (WB). GTX130377
Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377

Cat No. GTX130377

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Raji (1 mM pervanadate treatment for 30 min)

Predict Reactivity

Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human BTK (phospho Tyr223). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886249

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus

Background

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377

GTX130377 IHC-P Image

BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse thymus gland.
BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377

GTX130377 IHC-P Image

BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat lymph node.
BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-BTK (phospho Tyr223) antibody used in Western Blot (WB). GTX130377

GTX130377 WB Image

Untreated (–) and treated (+) Raji whole cell extracts (1 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:6000.

Anti-BTK (phospho Tyr223) antibody used in IHC (Paraffin sections) (IHC-P). GTX130377

GTX130377 IHC-P Image

BTK (phospho Tyr223) antibody detects BTK (phospho Tyr223) protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse spleen.
BTK (phospho Tyr223) stained by BTK (phospho Tyr223) antibody (GTX130377) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169