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BTK (phospho Tyr551) antibody

Anti-BTK (phospho Tyr551) antibody used in Western Blot (WB). GTX133592

Cat No. GTX133592

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

76 kDa. ( Note )

Positive Control

Raji (1 mM Pervanadate treatment for 30 min)

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.57 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human BTK (phospho Tyr551). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2887034

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Bruton tyrosine kinase , AGMX1 , AT , ATK , BPK , IGHD3 , IMD1 , PSCTK1 , XLA

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein , Nucleus , Cytoplasm , Membrane , Nucleus

Background

The protein encoded by this gene plays a crucial role in B-cell development. Mutations in this gene cause X-linked agammaglobulinemia type 1, which is an immunodeficiency characterized by the failure to produce mature B lymphocytes, and associated with a failure of Ig heavy chain rearrangement. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BTK (phospho Tyr551) antibody used in Western Blot (WB). GTX133592

GTX133592 WB Image

Untreated (–) and treated (+) Raji whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BTK (phospho Tyr551) antibody (GTX133592) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

There are currently no references for BTK (phospho Tyr551) antibody (GTX133592). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for BTK (phospho Tyr551) antibody (GTX133592). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169