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BUB1 antibody

Overexpression
Anti-BUB1 antibody used in Western Blot (WB). GTX107497
Anti-BUB1 antibody used in Western Blot (WB). GTX107497
Anti-BUB1 antibody used in Western Blot (WB). GTX107497
Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107497

Cat. No. GTX107497

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

A431 , 293T , BUB1-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949770

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase , BUB1A , BUB1L , hBUB1

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BUB1 antibody used in Western Blot (WB). GTX107497

GTX107497 WB Image

BUB1 antibody detects BUB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
5 % SDS-PAGE
BUB1 antibody (GTX107497) dilution: 1:1000

Anti-BUB1 antibody used in Western Blot (WB). GTX107497

GTX107497 WB Image

Sample (30 ug of whole cell lysate)

A: A431 (GTX27909)

7.5% SDS PAGE

GTX107497 diluted at 1:3000

Anti-BUB1 antibody used in Western Blot (WB). GTX107497

GTX107497 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with BUB1 antibody (GTX107497) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BUB1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX107497

GTX107497 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using BUB1(GTX107497) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

REFERENCE

Application Reference

Cheng T et al. JCI Insight 2024; 9 (4) Inhibiting centrosome clustering reduces cystogenesis and improves kidney function in autosomal dominant polycystic kidney disease.
Rodriguez-Rodriguez JA et al. Curr Biol 2018; 28 (21) : 3422-3429.e5 Distinct Roles of RZZ and Bub1-KNL1 in Mitotic Checkpoint Signaling and Kinetochore Expansion.
Application : WB
Reactivity : Human
Zhou H et al. Nat Commun 2016; 7 : 10151 Cep57 is a Mis12-interacting kinetochore protein involved in kinetochore targeting of Mad1-Mad2.
Application : ICC/IF
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169