GeneTex
United States (US)

BUB1 antibody

GTX107497_39897_WB_2_19011506_105.jpg
GTX107497_39897_IFA_19011506_615.jpg
GTX107497_19011506_151.jpg
GTX107497_39897_20181102_WB_B_19011519_673.jpg

Cat No. GTX107497

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

Positive Control

293T , A431 , H1299 , HepG2 , Molt-4 , Raji

Predict Reactivity

Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.63 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human BUB1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bub1 Mitotic Checkpoint Serine/Threonine Kinase,Bub1A,Bub1L,Hbub1,Bub1

Cellular Localization

Nucleus , Kinetochore

Background

This gene encodes a kinase involved in spindle checkpoint function. The kinase functions in part by phosphorylating a member of the miotic checkpoint complex and activating the spindle checkpoint. Mutations in this gene have been associated with aneuploidy and several forms of cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX107497_39897_WB_2_19011506_105.jpg

GTX107497 WB Image

BUB1 antibody detects BUB1 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
5 % SDS-PAGE
BUB1 antibody (GTX107497) dilution: 1:1000

GTX107497_39897_IFA_19011506_615.jpg

GTX107497 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using BUB1(GTX107497) antibody (Geen) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

GTX107497_19011506_151.jpg

GTX107497 WB Image

Sample (30 ug of whole cell lysate)

A: A431 (GTX27909)

7.5% SDS PAGE

GTX107497 diluted at 1:3000

GTX107497_39897_20181102_WB_B_19011519_673.jpg

GTX107497 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with BUB1 antibody (GTX107497) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169