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BUB1 antibody

Anti-BUB1 antibody used in Western Blot (WB). GTX30097
Anti-BUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30097
Anti-BUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30097

Cat. No. GTX30097

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all BUB1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

122 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human BUB1 (NP_004327.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase , BUB1A , BUB1L , hBUB1

Cellular Localization

Nucleus

Background

This gene encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2013]

Database

Research Area

DATA IMAGES

Anti-BUB1 antibody used in Western Blot (WB). GTX30097

GTX30097 WB Image

WB analysis of various sample lysates using GTX30097 BUB1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30097

GTX30097 IHC-P Image

IHC-P analysis of mouse testis tissue using GTX30097 BUB1 antibody.
Dilution : 1:200

Anti-BUB1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30097

GTX30097 IHC-P Image

IHC-P analysis of rat liver tissue using GTX30097 BUB1 antibody.
Dilution : 1:200

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399