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BUB1B antibody [11G2]

Anti-BUB1B antibody [11G2] used in Western Blot (WB). GTX84768
Anti-BUB1B antibody [11G2] used in IHC (Paraffin sections) (IHC-P). GTX84768
Anti-BUB1B antibody [11G2] used in IHC (Paraffin sections) (IHC-P). GTX84768
Anti-BUB1B antibody [11G2] used in Western Blot (WB). GTX84768
Anti-BUB1B antibody [11G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84768

Cat. No. GTX84768

Host

Mouse

Clonality

Monoclonal

Clone Name

11G2

Isotype

IgG1

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Monkey
Package
100 μl ($519)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000
ICC/IF 1:100
IHC-P 1:50
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human BUB1B expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase B , BUB1beta , BUBR1 , Bub1A , MAD3L , MVA1 , SSK1 , hBUBR1

Cellular Localization

Cytoplasm,Nucleus

Background

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Database

Research Area

DATA IMAGES

Anti-BUB1B antibody [11G2] used in Western Blot (WB). GTX84768

GTX84768 WB Image

WB analysis of HEK293T cells transfected with BUB1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84768 BUB1B antibody [11G2].
Loading : 5 ug per lane

Anti-BUB1B antibody [11G2] used in IHC (Paraffin sections) (IHC-P). GTX84768

GTX84768 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84768 BUB1B antibody [11G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-BUB1B antibody [11G2] used in IHC (Paraffin sections) (IHC-P). GTX84768

GTX84768 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84768 BUB1B antibody [11G2].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-BUB1B antibody [11G2] used in Western Blot (WB). GTX84768

GTX84768 WB Image

WB analysis of various cell lines using GTX84768 BUB1B antibody [11G2].
Loading : 35 ug per lane

Anti-BUB1B antibody [11G2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84768

GTX84768 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84768 BUB1B antibody [11G2].

REFERENCE

There are currently no references for BUB1B antibody [11G2] (GTX84768). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for BUB1B antibody [11G2] (GTX84768). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 519