BUB1B antibody [5D9]

Cat. No. GTX84771

Reference 1

Host	Mouse
Clonality	Monoclonal
Clone Name	5D9
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, IHC
Reactivity	Human, Monkey
Package	100 μl ($519)

See all BUB1B products

View product citations for antibody GTX84771 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1:2000
ICC/IF	1:100
IHC-P	1:50
IHC	Assay dependent

Not tested in other applications.

Calculated MW

120 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human BUB1B expression vector

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_10729848

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase B , BUB1beta , BUBR1 , Bub1A , MAD3L , MVA1 , SSK1 , hBUBR1

Cellular Localization

Cytoplasm,Nucleus

Background

Essential component of the mitotic checkpoint. Required for normal mitosis progression. The mitotic checkpoint delays anaphase until all chromosomes are properly attached to the mitotic spindle. One of its checkpoint functions is to inhibit the activity of the anaphase-promoting complex/cyclosome (APC/C) by blocking the binding of CDC20 to APC/C, independently of its kinase activity. The other is to monitor kinetochore activities that depend on the kinetochore motor CENPE. Required for kinetochore localization of CENPE. Negatively regulates PLK1 activity in interphase cells and suppresses centrosome amplification. Also implicated in triggering apoptosis in polyploid cells that exit aberrantly from mitotic arrest. May play a role for tumor suppression.

Database

Gene ID: 701 BUB1B
UniProt: O60566 BUB1B

Research Area

DATA IMAGES

GTX84771 IHC-P Image

IHC-P analysis of colon adenocarcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of bladder carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with BUB1B plasmid using GTX84771 BUB1B antibody [5D9].

GTX84771 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of lung carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of liver carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of prostate carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 WB Image

WB analysis of HEK293T cells transfected with BUB1B plasmid (Right) or empty vector (Left) for 48 hrs using GTX84771 BUB1B antibody [5D9].
Loading : 5 ug per lane

GTX84771 WB Image

WB analysis of various cell lines using GTX84771 BUB1B antibody [5D9].
Loading : 35 ug per lane

GTX84771 IHC-P Image

IHC-P analysis of pancreas carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

GTX84771 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84771 BUB1B antibody [5D9].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Cirak Y et al. Int J Clin Exp Pathol 2015; 8 (5) : 5345-53 Predictive and prognostic values of BubR1 and synuclein-gamma expression in breast cancer.

Application : IHC

Reactivity : Human

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REVIEW

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Application Reference

Cirak Y et al. Int J Clin Exp Pathol 2015; 8 (5):5345-53 Predictive and prognostic values of BubR1 and synuclein-gamma expression in breast cancer.

Application : IHC

Reactivity : Human

SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μl

$ 519

Qty

BUB1B antibody [5D9]

Cat. No. GTX84771

Host

Clonality

Clone Name

Isotype

Application

Reactivity

APPLICATION

Application Note

Calculated MW

PROPERTIES

Form

Buffer

Preservative

Storage

Concentration

Antigen Species

Immunogen

Purification

Conjugation

RRID

Note

TARGET

Synonyms

Cellular Localization

Background

Database

Research Area

DATA IMAGES

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 ICC/IF Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 WB Image

GTX84771 WB Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

GTX84771 IHC-P Image

REFERENCE

Application Reference

REVIEW

Product

Research

Support

Company

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