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BUB1B antibody [N2C1], Internal

Anti-BUB1B antibody [N2C1], Internal used in Western Blot (WB). GTX111289
Anti-BUB1B antibody [N2C1], Internal used in Western Blot (WB). GTX111289
Anti-BUB1B antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111289

Cat. No. GTX111289

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all BUB1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

Positive Control

K562 , 293T , HeLa , HepG2

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BUB1B. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949771

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase B , BUB1beta , BUBR1 , Bub1A , MAD3L , MVA1 , SSK1 , hBUBR1

Cellular Localization

Cytoplasm , Nucleus , Kinetochore

Background

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BUB1B antibody [N2C1], Internal used in Western Blot (WB). GTX111289

GTX111289 WB Image

Whole cell extract (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal (GTX111289) diluted at 1:500.

Anti-BUB1B antibody [N2C1], Internal used in Western Blot (WB). GTX111289

GTX111289 WB Image

Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with BUB1B antibody [N2C1], Internal (GTX111289) diluted at 1:500.

Anti-BUB1B antibody [N2C1], Internal used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111289

GTX111289 ICC/IF Image

The data was published in the journal Elife in 2018. PMID: 29517484

REFERENCE

Application Reference

Wang SJ et al. J Pers Med 2021; 11 (5) A Bioinformatics Analysis Identifies the Telomerase Inhibitor MST-312 for Treating High-STMN1-Expressing Hepatocellular Carcinoma.
Application : WB
Reactivity : Human
Lee CC et al. Elife 2018; 7 Sumoylation promotes optimal APC/C activation and timely anaphase.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169