GeneTex

  • Country / Location Selection

United States (US)

BUB1B antibody

Anti-BUB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55541
Anti-BUB1B antibody used in Western Blot (WB). GTX55541
Anti-BUB1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55541

Cat. No. GTX55541

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Monkey
Package
100 μl ($399)
See all BUB1B products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

120 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human BUB1B (NP_001202.4).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB1 mitotic checkpoint serine/threonine kinase B , BUB1beta , BUBR1 , Bub1A , MAD3L , MVA1 , SSK1 , hBUBR1

Cellular Localization

Cytoplasm,Nucleus

Background

This gene encodes a kinase involved in spindle checkpoint function. The protein has been localized to the kinetochore and plays a role in the inhibition of the anaphase-promoting complex/cyclosome (APC/C), delaying the onset of anaphase and ensuring proper chromosome segregation. Impaired spindle checkpoint function has been found in many forms of cancer. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BUB1B antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55541

GTX55541 ICC/IF Image

ICC/IF analysis of NIH-3T3 cells using GTX55541 BUB1B antibody.
Blue : DAPI
Dilution : 1:100

Anti-BUB1B antibody used in Western Blot (WB). GTX55541

GTX55541 WB Image

WB analysis of various sample lysates using GTX55541 BUB1B antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-BUB1B antibody used in IHC (Paraffin sections) (IHC-P). GTX55541

GTX55541 IHC-P Image

IHC-P analysis of human breast cancer tissue using GTX55541 BUB1B antibody.
Dilution : 1:200

REFERENCE

There are currently no references for BUB1B antibody (GTX55541). Be the first to share your publications with this product.
Submit a Reference

REVIEW

There are currently no reviews for BUB1B antibody (GTX55541). Be the first to share your experience with this product.
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399