Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BUB3(GTX113595) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] (GTX113595).
Western blot analysis was performed using BUB3 antibody [N1C1] (GTX113595).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

BUB3 antibody detects BUB3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody (GTX113595) diluted by 1:1000.

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
10% SDS PAGE
GTX113595 diluted at 1:1000