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BUB3 antibody [N1C1]

IP/MS
Anti-BUB3 antibody [N1C1] used in Immunoprecipitation (IP). GTX113595
Anti-BUB3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113595
Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595
Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595
Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595
Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595

Cat. No. GTX113595

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IP

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all BUB3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

H1299 , 293T , A431 , HeLa , HepG2 , Neuro 2A , Raw264.7 , C2C12 , PC-12 , Rat2

Product Note

IP/MS validation was supported by references (PMID:30377413)

Predict Reactivity

Zebrafish, Bovine, Chicken, Xenopus tropicalis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.32 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human BUB3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036374

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BUB3 mitotic checkpoint protein , BUB3L , hBUB3

Cellular Localization

Nucleus

Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-BUB3 antibody [N1C1] used in Immunoprecipitation (IP). GTX113595

GTX113595 IP Image

Immunoprecipitation of BUB3 protein from 293T whole cell extracts using 5 μg of BUB3 antibody [N1C1] (GTX113595).
Western blot analysis was performed using BUB3 antibody [N1C1] (GTX113595).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-BUB3 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX113595

GTX113595 IHC-P Image

Immunohistochemical analysis of paraffin-embedded A549 Xenograft, using BUB3(GTX113595) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595

GTX113595 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595

GTX113595 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
B: H1299
10% SDS PAGE
GTX113595 diluted at 1:1000

Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595

GTX113595 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody [N1C1] (GTX113595) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-BUB3 antibody [N1C1] used in Western Blot (WB). GTX113595

GTX113595 WB Image

BUB3 antibody detects BUB3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with BUB3 antibody (GTX113595) diluted by 1:1000.

REFERENCE

Application Reference

Li Q et al. Adv Sci (Weinh) 2024; 11 (35) : e2406009 CCDC68 Maintains Mitotic Checkpoint Activation by Promoting CDC20 Integration into the MCC.
Sikorski K et al. Nat Methods 2018; 15 (11) : 909-912 A high-throughput pipeline for validation of antibodies.
Application : IP
Reactivity : Human
Chang JF et al. Toxicol Sci 2014; (Epub) nuclear proteomics with xrcc3 knockdown to reveal the development of *-resistant uterine cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169