GeneTex
United States (US)

BUB3 antibody

GTX31314_118_ICC-IF_20180221_18121410_223.jpg
GTX31314_119_ICC-IF_20180221_18121410_555.jpg
GTX31314_2518_WB_20180221_18121410_591.jpg

Cat No. GTX31314

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, ELISA
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 1 μg/mL
ICC/IF 10 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

Positive Control

Jurkat Cell Slide(GTX25533) , Jurkat Cell Lysate(GTX27899)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bub3 antibody was raised against a 16 amino acid synthetic peptide from near the carboxy terminus of human bub3.The immunogen is located within the last 50 amino acids of Bub3.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bub3, Mitotic Checkpoint Protein,Bub3L,Hbub3,Bub3

Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31314_118_ICC-IF_20180221_18121410_223.jpg

GTX31314 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31314 BUB3 antibody.
Working concentration : 10 μg/ml

GTX31314_119_ICC-IF_20180221_18121410_555.jpg

GTX31314 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX31314 BUB3 antibody.
Working concentration : 20 μg/ml

GTX31314_2518_WB_20180221_18121410_591.jpg

GTX31314 WB Image

WB analysis of Jurkat cell lysate using GTX31314 BUB3 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml

Package List Price ($)
$ 329