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BUB3 antibody

Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33047
Anti-BUB3 antibody used in Western Blot (WB). GTX33047

Cat No. GTX33047

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human, Mouse
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
Not tested in other applications.

Calculated MW

37 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-328 of human BUB3 (NP_004716.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BUB3 mitotic checkpoint protein , BUB3L , hBUB3

Cellular Localization

Nucleus

Background

This gene encodes a protein involved in spindle checkpoint function. The encoded protein contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-BUB3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33047

GTX33047 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX33047 BUB3 antibody.

Anti-BUB3 antibody used in Western Blot (WB). GTX33047

GTX33047 WB Image

WB analysis of various samples using GTX33047 BUB3 antibody.The signal was developed with ECL plus-Enhanced.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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Package List Price ($)
$ 329