GeneTex
United States (US)

Baboon antibody

Baboon-antibody_WB_GTX14681-1_18121409_221.jpg

Cat No. GTX14681

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IP
Reactivity Drosophila
APPLICATION

Application Note

ICC: Use at an assay dependent dilution. IP: Use at a dilution of >1/200. WB: Use at a dilution of >1/500. Detects a band of approximately 73 kDa Optimal dilutions/concentrations should be determined by the end user.

Positive Control

D. melanogaster extracts

Specificity/Sensitivity

The antibody is unique to Baboon protein
PROPERTIES

Form

Liquid

Buffer

Preservative: NoneConstituents: BSA, Glycerol, Ethalene glycol. pH 7.4

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.75-1.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Drosophila

Immunogen

Synthetic peptide: CNKNENMKRIFETDYVPHRLTS, corresponding to N terminal amino acids 200-221 of Drosophilia melanogaster Baboon. The peptide was amidated for conjugation.

Purification

Immunogen affinity purified
Purified against immobilized antigen based affinity chromatography which yielded an epitope specific antibody.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Baboon,Atr-1,Atr-I,Atf1,Atr-1,Atr-I,Atr45A,Atri,Babo,Bab,Babo,Babo-A,Cg8224,Dmel\Cg8224,Gprk-3,Gprk3,Stk-E,Atr-I,L(2)44Fd,L(2)K16912,Babo

Background

Transmembrane receptor Patched regulates several developmental processes in both invertebrates and vertebrates. In higher vertebrates the patched (Ptc) also acts as a tumor suppressor. The patched receptor signaling involves negatively regulating a GPCR, smoothened. The binding Hedgehog ligand to Ptc removes the negative interaction of Ptc, and allows the signaling by smoothened. It has been shown that during head morphogenesis in D. melanogaster, Ptc positively interacts with smoothened, which leads to stimulation of Activin type 1 receptor Baboon, and activation of cell proliferation in the eye antennal disc. The expression of activated Baboon in the Ptc domain in Ptc mutant background completely abolish the head developmental defects.

Research Area

DATA IMAGES
Baboon-antibody_WB_GTX14681-1_18121409_221.jpg

GTX14681 WB Image

Western blot of BaboonA with Baboon antibody GTX14681.
Lane 1,2: cells expressing BaboonA
Lane 3: mock transfected cells

Package List Price ($)
$ 319