ELISA analysis of crude extract of B. cereus using GTX00866 Bacillus cereus phospholipase C antibody [bc-01].
Protein coating amount : 100μl per well at indicated amount
Antibody amount : 100μl (1:500) per well

Reactivities of GTX00866 Bacillus cereus phospholipase C antibody [bc-01] with various food poisoning bacter. GTX00866 reacts with a standard strain of B. cereus (NBRC15306), 5 isolated strains, and both PLC of B. cereus and α-toxin of C. perfringens. Antibody reacts with C. perfringens, but not with any other food poisoning bacteria. Protein bands, 28 kDa and 43kDa sizes correspond to the expected sizes of the B. cereus PLC and C. peringens α-toxin, respectively.

ELISA analysis of crude extract of B. cereus using GTX00866 Bacillus cereus phospholipase C antibody [bc-01].
Protein coating amount : 100μl (1 μg/ml) per well
Antibody amount : 100μl at indicated dilution

WB analysis of various samples using GTX00866 Bacillus cereus phospholipase C antibody [bc-01].
Lane 1 : Culture medium of B. cereus
Lane 2 : PC-PLC purified from B. cereus
Dilution : 1:1000