GeneTex
United States (US)

Bactin1 antibody

GTX124388_42809_20170425_IHC-Wm_Z_18121410_615.jpg
GTX124388_42032_20150909_IHC-Wm_Z_18121410_866.jpg
GTX124388_42894_20170704_IHC-Wm_Z_18121410_187.jpg
GTX124388_42809_20170511_WB_Z_18121410_994.jpg
GTX124388_40219_IHC_Z_18121410_561.jpg

Cat No. GTX124388

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr, IHC-Wm
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

zebrafish muscle , whole zebrafish
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Beta 1,Actb,B-Actzf,Actba,Bact,Bactin1,Bactzf,Wu:Fd18F05,Actb1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES
GTX124388_42809_20170425_IHC-Wm_Z_18121410_615.jpg

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:100.

GTX124388_42032_20150909_IHC-Wm_Z_18121410_866.jpg

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:100.

GTX124388_42894_20170704_IHC-Wm_Z_18121410_187.jpg

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:50.

GTX124388_42809_20170511_WB_Z_18121410_994.jpg

GTX124388 WB Image

Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124388) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124388_40219_IHC_Z_18121410_561.jpg

GTX124388 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using bactin1 (GTX124388) antibody at 1:300 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199