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Bactin1 antibody

Anti-Bactin1 antibody used in Western Blot (WB). GTX124388
Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388
Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388
Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388
Anti-Bactin1 antibody used in Western Blot (WB). GTX124388

Cat. No. GTX124388

Reference 4

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-Fr, IHC-Wm

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Bactin1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5000-1:20000
IHC-Fr Assay dependent
IHC-Wm 1:50-1:500
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

zebrafish muscle , whole zebrafish

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Rabbit (99%)| Sheep (100%)| Bovine (100%)| Dog (100%)| Chicken (100%)| Guinea pig (100%)| Xenopus tropicalis (99%)| Chimpanzee (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.05 mg/ml (Please refer to the vial label for the specific concentration.)

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Bactin1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11177450

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin, beta 1 , ACTB , B-ACTZF , actba , bact , bactin1 , bactzf , wu:fd18f05

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES

Anti-Bactin1 antibody used in Western Blot (WB). GTX124388

GTX124388 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124388) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: 2 days-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:100.
Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on zebrafish by whole mount immunohistochemical analysis.
Sample: Paraformaldehyde-fixed 2 day-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:100.

Anti-Bactin1 antibody used in IHC (Whole mount) (IHC-Wm). GTX124388

GTX124388 IHC-Wm Image

Bactin1 antibody detects Bactin1 protein on whole mount zebrafish by immunohistochemical analysis.
Sample: 2 day-post-fertilization zebrafish embryo.
Bactin1 antibody (GTX124388) dilution: 1:50.

Anti-Bactin1 antibody used in Western Blot (WB). GTX124388

GTX124388 WB Image

The data was published in the journal Front Mol Neurosci in 2019.PMID: 31178691

REFERENCE

Application Reference

Borna NN et al. Genes (Basel) 2020; 11 (11) Leigh Syndrome Due to NDUFV1 Mutations Initially Presenting as LBSL.
Li G et al. Front Mol Neurosci 2019; 12 : 126 Tubgcp3 Is Required for Retinal Progenitor Cell Proliferation During Zebrafish Development.
Liu P et al. Biotechnol Lett 2017; (Epub) MAPK/ERK signalling is required for zebrafish cardiac regeneration.
Application : IHC-Fr
Reactivity : Zebrafish
Zhao LJ et al. Vet Microbiol 2013; 166 (43102) : 233-41 Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri.
Application : WB
Reactivity : Zebrafish
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169