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Bactin1 antibody

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500
Anti-Bactin1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124500
Anti-Bactin1 antibody used in Western Blot (WB). GTX124500
Anti-Bactin1 antibody used in Western Blot (WB). GTX124500
Anti-Bactin1 antibody used in Western Blot (WB). GTX124500
Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

Cat. No. GTX124500

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all zebrafish Bactin1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

zebrafish heart , zebrafish muscle , whole zebrafish

Predict Reactivity

Human (100%)| Mouse (100%)| Rat (100%)| Rabbit (100%)| Sheep (100%)| Bovine (100%)| Dog (100%)| Chicken (100%)| Guinea pig (100%)| Xenopus tropicalis (100%)| Chimpanzee (100%)| Rhesus Monkey (100%)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Zebrafish

Immunogen

The immunogen used to generate this antibody corresponds to zebrafish Bactin1

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11167400

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

actin, beta 1 , ACTB , B-ACTZF , actba , bact , bactin1 , bactzf , wu:fd18f05

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

GTX124500 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124500) diluted at 1:1000.

Anti-Bactin1 antibody used in IHC (Paraffin sections) (IHC-P). GTX124500

GTX124500 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using bactin1 (GTX124500) antibody at 1:300 dilution.

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

GTX124500 WB Image

The data was published in the journal Int J Mol Sci in 2017. PMID: 28534853

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

GTX124500 WB Image

The data was published in the journal Int J Mol Sci in 2017. PMID: 28534853

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

GTX124500 WB Image

The data was published in the journal Int J Mol Sci in 2017. PMID: 28534853

Anti-Bactin1 antibody used in Western Blot (WB). GTX124500

GTX124500 WB Image

The data was published in the journal Int J Mol Sci in 2017. PMID: 28534853

REFERENCE

Application Reference

Kao CJ et al. Int J Mol Sci 2017; 18 (5) The 1-Tosylpentan-3-one Protects against 6-Hydroxydopamine-Induced Neurotoxicity.
Application : WB
Reactivity : Zebrafish
Huang SY et al. PLoS One 2014; 9 (8) : e104414 A novel zebrafish model to provide mechanistic insights into the inflammatory events in carrageenan-induced abdominal edema.
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169