GeneTex
United States (US)

Bactin1 antibody

GTX124501_42305_20151203_WB_Z_18121410_548.jpg
GTX124501_42753_20170615_WB_Z_18121410_306.jpg
GTX124501_40177_IHC_18121410_832.jpg

Cat No. GTX124501

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

zebrafish heart , zebrafish muscle , whole zebrafish
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.53 mg/ml (Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Actin, Beta 1,Actb,B-Actzf,Actba,Bact,Bactin1,Bactzf,Wu:Fd18F05,Actb1

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES
GTX124501_42305_20151203_WB_Z_18121410_548.jpg

GTX124501 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124501) diluted at 1:5000.

GTX124501_42753_20170615_WB_Z_18121410_306.jpg

GTX124501 WB Image

Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124501) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124501_40177_IHC_18121410_832.jpg

GTX124501 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using bactin1 (GTX124501) antibody at 1:300 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199