GeneTex
United States (US)

Bactin1 antibody

GTX124501_42305_20151203_WB_Z_18121410_548.jpg
GTX124501_42753_20170615_WB_Z_18121410_306.jpg
GTX124501_40177_IHC_18121410_832.jpg

Cat No. GTX124501

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Zebrafish
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:1000-1:10000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

42 kDa. ( Note )

Positive Control

zebrafish heart , zebrafish muscle , whole zebrafish
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

2.53mg/ml(Please refer to the vial label for the specific concentration.)

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

beta-actin-1 antibody, beta-actin antibody, "actin, cytoplasmic 1 antibody", ACTB antibody, B-ACTZF antibody, actba antibody, bact antibody, bactzf antibody, wu:fd18f05 antibody, bactin1 antibody

Cellular Localization

Cytoplasm , cytoskeleton

Background

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

Database

Research Area

DATA IMAGES
GTX124501_42305_20151203_WB_Z_18121410_548.jpg

GTX124501 WB Image

Various tissue extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124501) diluted at 1:5000.

GTX124501_42753_20170615_WB_Z_18121410_306.jpg

GTX124501 WB Image

Various tissue extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Bactin1 antibody (GTX124501) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124501_40177_IHC_18121410_832.jpg

GTX124501 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using bactin1 (GTX124501) antibody at 1:300 dilution.

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 389
$ 199