GeneTex
United States (US)

Bad antibody

GTX31688_2856_WB_20180221_18121410_205.jpg
GTX31688_1345_IHC-P_20180221_18121410_832.jpg
GTX31688_277_IHC-P_20180221_18121410_349.jpg

Cat No. GTX31688

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, ELISA
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5 - 2 μg/mL
IHC-P 2 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Positive Control

T24 Cell Lysate(GTX23958) , Rat Thymus Tissue Lysate(GTX24041)
PROPERTIES

Form

Liquid

Buffer

PBS with 0.02% sodium azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Bad antibody was raised against a peptide corresponding to 15 amino acids near the C-terminus of human Bad.The immunogen is located within amino acids 90 - 140 of BAD.

Purification

Purified by ion exchange chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated Agonist Of Cell Death,Bbc2,Bcl2L8,Bad

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX31688_2856_WB_20180221_18121410_205.jpg

GTX31688 WB Image

WB analysis of T24 cell lysate using GTX31688 Bad antibody.
Working concentration : (A) 0.5, (B) 1, and (C) 2 μg/ml

GTX31688_1345_IHC-P_20180221_18121410_832.jpg

GTX31688 IHC-P Image

IHC-P analysis of rat thymus tissue using GTX31688 Bad antibody.
Working concentration : 2 μg/ml

GTX31688_277_IHC-P_20180221_18121410_349.jpg

GTX31688 IHC-P Image

IHC-P analysis of rat thymus tissue using GTX31688 Bad antibody.
Working concentration : 10 μg/ml