GeneTex
United States (US)

Bad (phospho Ser112) antibody

GTX32370_20190131_IHC-P_19013102_396.jpg
GTX32370_20190131_WB_19013102_806.jpg

Cat No. GTX32370

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of Bad. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated Agonist Of Cell Death,Bbc2,Bcl2L8,Bad

Cellular Localization

Cytoplasm,Mitochondrion outer membrane

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
GTX32370_20190131_IHC-P_19013102_396.jpg

GTX32370 IHC-P Image

IHC-P analysis of formalin fixed human colon cancer tissue section using GTX32370 Bad (phospho Ser112) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

GTX32370_20190131_WB_19013102_806.jpg

GTX32370 WB Image

WB analysis of Forskolin-treated HeLa (A), MCF7 (B) whole cell lysates using GTX32370 Bad (phospho Ser112) antibody.