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Bad (phospho Ser136) antibody

Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136
Anti-Bad (phospho Ser136) antibody used in IHC (Paraffin sections) (IHC-P). GTX50136

Cat No. GTX50136

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P, IP

Reactivity

Human, Mouse
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:1000
IHC-P 1:50-1:100
IP Assay dependent
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Predict Reactivity

Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH 7.4, 150mM NaCl, 0.02% sodium azide, 50% glycerol

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Peptide sequence around phosphorylation site of serine136 (S-R-S(p)-A-P) derived from mouse Bad.

Purification

Purified by antigen-affinity chromatography. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2-associated agonist of cell death , AI325008 , Bbc2

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Database

Research Area

DATA IMAGES

Anti-Bad (phospho Ser136) antibody used in Western Blot (WB). GTX50136

GTX50136 WB Image

WB analysis of extracts from HT-29 cells untreated or treated with UV using GTX50136 Bad (phospho Ser136) antibody.

Anti-Bad (phospho Ser136) antibody used in IHC (Paraffin sections) (IHC-P). GTX50136

GTX50136 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX50136 Bad (phospho Ser136) antibody.
Left : Primary antibody
Right : Primary antibody pre-incubated with the antigen specific peptide

REFERENCE

REVIEW

Bad (phospho Ser136) antibody Cat No. GTX50136

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 19-Nov-2020
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was pear reviewed and published in the journal Front Mol Neurosci in 2017.PMID: 28676741
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf