GeneTex
  • Country / Location Selection

United States (US)

Bad (phospho Ser155) antibody

Anti-Bad (phospho Ser155) antibody used in Western Blot (WB). GTX32371
Anti-Bad (phospho Ser155) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32371
Anti-Bad (phospho Ser155) antibody used in IHC (Paraffin sections) (IHC-P). GTX32371

Cat No. GTX32371

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($319)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium phosphate, 0.87% Sodium chloride pH 7.3, 30% glycerol, 0.01% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20º C or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of Bad. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 associated agonist of cell death , BBC2 , BCL2L8

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bad (phospho Ser155) antibody used in Western Blot (WB). GTX32371

GTX32371 WB Image

WB analysis of Forskolin-treated HeLa (A) whole cell lysates using GTX32371 Bad (phospho Ser155) antibody.

Anti-Bad (phospho Ser155) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32371

GTX32371 ICC/IF Image

ICC/IF analysis of formalin-fixed Hela cells using GTX32371 Bad (phospho Ser155) antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

Anti-Bad (phospho Ser155) antibody used in IHC (Paraffin sections) (IHC-P). GTX32371

GTX32371 IHC-P Image

IHC-P analysis of formalin fixed human prostate cancer tissue section using GTX32371 Bad (phospho Ser155) antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

REFERENCE

There are currently no references for Bad (phospho Ser155) antibody (GTX32371). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Bad (phospho Ser155) antibody (GTX32371). Be the first to share your experience with this product.
Package List Price ($)
$ 319