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Bad (phospho Ser155) antibody

Anti-Bad (phospho Ser155) antibody used in IHC (Paraffin sections) (IHC-P). GTX79126
Anti-Bad (phospho Ser155) antibody used in Western Blot (WB). GTX79126

Cat No. GTX79126

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:1000
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

18 kDa. ( Note )

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 155 (R-M-Sp-D-E).

Purification

Purified by sequential chromatography on phospho- and non-phospho-peptide affinity columns.
From serum

Conjugation

Unconjugated

RRID

AB_11179683

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BAD,BBC2,BCL2 associated agonist of cell death,BCL2L8,Bad

Cellular Localization

Mitochondrion outer membrane,Cytoplasm

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bad (phospho Ser155) antibody used in IHC (Paraffin sections) (IHC-P). GTX79126

GTX79126 IHC-P Image

IHC-P analysis of human breast carcinoma tissue using GTX79126 Bad (phospho Ser155) antibody.

Anti-Bad (phospho Ser155) antibody used in Western Blot (WB). GTX79126

GTX79126 WB Image

WB analysis of 293 cell lysate using GTX79126 Bad (phospho Ser155) antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 339