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Bag1 antibody [N3C3]

Anti-Bag1 antibody [N3C3] used in Western Blot (WB). GTX102433
Anti-Bag1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102433

Cat No. GTX102433

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat, Bovine, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human Bag1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 associated athanogene 1 , BAG-1 , HAP , RAP46

Cellular Localization

Isoform 2: Cytoplasm , Nucleus

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. At least three protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) start site, and alternative, downstream, AUG translation initiation sites. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bag1 antibody [N3C3] used in Western Blot (WB). GTX102433

GTX102433 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
10% SDS PAGE
Bag1 antibody
GTX102433 diluted at 1:1000

Anti-Bag1 antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102433

GTX102433 ICC/IF Image

Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Bag1(GTX102433) antibody at 1:200 dilution.

REFERENCE

There are currently no references for Bag1 antibody [N3C3] (GTX102433). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for Bag1 antibody [N3C3] (GTX102433). Be the first to share your experience with this product.
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169