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Bag1 antibody

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082
Anti-Bag1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30082
Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082
Anti-Bag1 antibody used in Western Blot (WB). GTX30082
Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082

Cat. No. GTX30082

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Bag1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887592

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 1 , BAG-1 , HAP , RAP46

Cellular Localization

Cytoplasm,Nucleus

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082

GTX30082 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

Anti-Bag1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX30082

GTX30082 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX30082 Bag1 antibody.
Blue : DAPI

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082

GTX30082 IHC-P Image

IHC-P analysis of human kidney damage tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

Anti-Bag1 antibody used in Western Blot (WB). GTX30082

GTX30082 WB Image

WB analysis of various sample lysates using GTX30082 Bag1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX30082

GTX30082 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

REFERENCE

Application Reference

Lin PL et al. Eur J Cancer 2017; 85 : 95-105 An increase in BAG-1 by PD-L1 confers resistance to tyrosine kinase inhibitor in non-small cell lung cancer via persistent activation of ERK signalling.
Application : IHC-P
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399