GeneTex
United States (US)

Bag1 antibody

GTX30082_20180530_IHC-P_3_18121410_914.jpg
GTX30082_20180530_IHC-P_2_18121410_660.jpg
GTX30082_20180530_ICCIF_18121410_388.jpg
GTX30082_20180530_IHC-P_1_18121410_477.jpg
GTX30082_20180530_WB_18121410_604.jpg
GTX30082_20180530_IHC-P_18121410_869.jpg

Cat No. GTX30082

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500 - 1:2000
ICC/IF 1:20 - 1:100
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

39 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

PBS pH7.3, 0.02% sodium azide, 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-230 of human BAG1 (NP_001165886.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated Athanogene 1,Bag-1,Hap,Rap46,Bag1

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES
GTX30082_20180530_IHC-P_3_18121410_914.jpg

GTX30082 IHC-P Image

IHC-P analysis of human kidney injury tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

GTX30082_20180530_IHC-P_2_18121410_660.jpg

GTX30082 IHC-P Image

IHC-P analysis of human kidney cancer tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

GTX30082_20180530_ICCIF_18121410_388.jpg

GTX30082 ICC/IF Image

ICC/IF analysis of U2OS cell using GTX30082 Bag1 antibody.
Blue : DAPI

GTX30082_20180530_IHC-P_1_18121410_477.jpg

GTX30082 IHC-P Image

IHC-P analysis of human esophagus tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

GTX30082_20180530_WB_18121410_604.jpg

GTX30082 WB Image

WB analysis of various samples using GTX30082 Bag1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

GTX30082_20180530_IHC-P_18121410_869.jpg

GTX30082 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX30082 Bag1 antibody.
Dilution : 1:100

REFERENCE
Package List Price ($)
$ 319