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Bag1 antibody

Anti-Bag1 antibody used in Western Blot (WB). GTX31736
Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31736
Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31736

Cat. No. GTX31736

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, ELISA

Reactivity

 Human, Mouse, Rat
Package
100 μg ($399)
See all Bag1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1 - 2 μg/mL
IHC-P 5 μg/mL
ELISA Assay dependent
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

Positive Control

PC-3 Cell Lysate(GTX23954)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

BAG-1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human BAG-1.The immunogen is located within the last 50 amino acids of BAG-1.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated athanogene 1 , BAG-1 , HAP , RAP46

Cellular Localization

Cytoplasm,Nucleus

Background

The oncogene BCL2 is a membrane protein that blocks a step in a pathway leading to apoptosis or programmed cell death. The protein encoded by this gene binds to BCL2 and is referred to as BCL2-associated athanogene. It enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms. Multiple protein isoforms are encoded by this mRNA through the use of a non-AUG (CUG) initiation codon, and three alternative downstream AUG initiation codons. A related pseudogene has been defined on chromosome X. [provided by RefSeq, Feb 2010]

Database

Research Area

DATA IMAGES

Anti-Bag1 antibody used in Western Blot (WB). GTX31736

GTX31736 WB Image

WB analysis of PC-3 cell lysate using GTX31736 Bag1 antibody.
Working concentration : (A) 1 and (B) 2 μg/ml

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31736

GTX31736 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31736 Bag1 antibody.
Working concentration : 5 μg/ml

Anti-Bag1 antibody used in IHC (Paraffin sections) (IHC-P). GTX31736

GTX31736 IHC-P Image

IHC-P analysis of mouse brain tissue using GTX31736 Bag1 antibody.
Working concentration : 20 μg/ml
Green : Primary antibody
Blue : DAPI
Red : Actin

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399