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Band3 antibody [BIII-136]

Cat. No. GTX11012

Reference 2

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 BIII-136

Isotype

 IgG2a

Application

 WB, ICC/IF, FACS, IP, ELISA

Reactivity

 Human
Package
100 μl ($469)
See all Band3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:5,000
ICC/IF Assay dependent
FACS Assay dependent
IP Assay dependent
ELISA Assay dependent
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

human erythrocytes

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

15mM Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Glycophorin B from human erythrocytes.

Purification

Unpurified

Conjugation

Unconjugated

RRID

AB_384205

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

solute carrier family 4 member 1 (Diego blood group) , AE1 , BND3 , CD233 , CHC , DI , EMPB3 , EPB3 , FR , RTA1A , SAO , SPH4 , SW , WD , WD1 , WR

Cellular Localization

Cell membrane

Background

The Band 3 protein is the most abundant integral protein of human erythrocyte membranes and functions as the major anion transporter polypeptide of erythrocytes. Band 3 is a 90-100 kD protein, its microheterogeneity is ascribed mainly to the structural heterogeneity of the carbohydrate moiety. The C-terminal portion of the molecule spans the membrane several times, and is responsible for anion transport. The N-terminal portion, comprising almost half of the polypeptide chain of Band 3, is located inside the red cell and interacts with cytoskeletal and cytoplasmic proteins such as ankyrin.

Database

Research Area

REFERENCE

Application Reference

Andolfo I et al. Blood Adv 2023; 7 (12) : 2681-2693 Proteome alterations in erythrocytes with PIEZO1 gain-of-function mutations.
Galletti J et al. J Immunol 2008; 181 (5) : 3674-83 Chronic lymphocytic leukemia cells bind and present the erythrocyte protein band 3: possible role as initiators of autoimmune hemolytic anemia.
Application : FACS
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 469