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Bassoon antibody [HL1732]

Anti-Bassoon antibody [HL1732] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637380
Anti-Bassoon antibody [HL1732] used in IHC (Paraffin sections) (IHC-P). GTX637380

Cat. No. GTX637380

Host

Rabbit

Clonality

Monoclonal

Clone Name

HL1732

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

419 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Synthetic peptide of mouse Bassoon

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

bassoon

Cellular Localization

Cytoplasm,Synapse, synaptosome,Cytoplasm, cytoskeleton

Background

A structural constituent of presynaptic active zone. Involved in presynapse to nucleus signaling pathway; protein localization to synapse; and regulation of synaptic vesicle cycle. Located in several cellular components, including glutamatergic synapse; postsynaptic density; and presynaptic active zone cytoplasmic component. Is active in GABA-ergic synapse and Schaffer collateral - CA1 synapse. Is expressed in nervous system and retina. Used to study epilepsy. Orthologous to human BSN (bassoon presynaptic cytomatrix protein). [provided by Alliance of Genome Resources, Apr 2022]

Database

Research Area

DATA IMAGES

Anti-Bassoon antibody [HL1732] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637380

GTX637380 ICC/IF Image

Bassoon antibody [HL1732] detects Bassoon protein at synapse by immunofluorescent analysis.
Sample: DIV9 rat E18 primary hippocampal neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bassoon stained by Bassoon antibody [HL1732] (GTX637380) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-Bassoon antibody [HL1732] used in IHC (Paraffin sections) (IHC-P). GTX637380

GTX637380 IHC-P Image

Bassoon antibody [HL1732] detects Bassoon protein at cell membrane and cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse cerebellum.
Bassoon stained by Bassoon antibody [HL1732] (GTX637380) diluted at 1:100.
Antigen Retrieval: Tris-EDTA buffer, pH 8.0, 15 min

REFERENCE

There are currently no references for Bassoon antibody [HL1732] (GTX637380). Be the first to share your publications with this product.

REVIEW

Bassoon antibody [HL1732] Cat. No. GTX637380

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 24-May-2023
Score :
Application Tested : WB
Sample Species : Hu
Sample : boiled/unboiled SH-SY5Y and SH-SY5Y membrane extract
Amount used : 30μg
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description :
WCL: whole cell lysate
ME: membrane extract
Package List Price ($)
$ 399
$ 169
Custom Conjugations