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Bax antibody [2D2]

Anti-Bax antibody [2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34422
Anti-Bax antibody [2D2] used in Flow cytometry (FACS). GTX34422
Anti-Bax antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX34422

Cat. No. GTX34422

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 2D2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, FACS

Reactivity

 Human, Monkey
Package
100 μg ($529)
See all Bax products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1-2μg/ml
ICC/IF 1-2μg/ml
IHC-P 1-2μg/ml for 30 minutes at RT
FACS 1-2μg/10⁶ cells

Note :

IHC-P
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

10mM PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAX , BCL2 associated X, apoptosis regulator , BCL2L4 , Bax

Cellular Localization

Mitochondrion membrane,Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bax antibody [2D2] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX34422

GTX34422 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX34422 Bax antibody [2D2].
Green : Primary antibody
Red : nucleus

Anti-Bax antibody [2D2] used in Flow cytometry (FACS). GTX34422

GTX34422 FACS Image

FACS analysis of Jurkat cells using GTX34422 Bax antibody [2D2].
Blue : Blank
Red : Isotype control
Orange : Primary antibody

Anti-Bax antibody [2D2] used in IHC (Paraffin sections) (IHC-P). GTX34422

GTX34422 IHC-P Image

IHC-P analysis of human Hodgkin's lymphoma tissue using GTX34422 Bax antibody [2D2].

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 529