GeneTex
United States (US)

Bax antibody [2D3]

Bax-antibody-2D3_WB_GTX16119-1_18121409_882.jpg

Cat No. GTX16119

Host Mouse
Clonality Monoclonal
Clone Name 2D3
Isotype IgG1
Application WB
Reactivity Human, Rat, Bovine, Cat, Dog
APPLICATION

Application Note

WB: 1/10000. Predicted molecular weight: 21 kDa. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

21 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Synthetic peptide: MDGSGEQPRGGGPTSS, corresponding to amino acids 3-16 of Human Bax.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated X, Apoptosis Regulator,Bcl2L4,Bax

Cellular Localization

Cell Membrane

Background

Bcl2 family is a key regulator of apoptosis that functions to either inhibit or promote cell death. Over-expression of members such as Bcl2 and BclxL inhibit the apoptotic process. The Bcl2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with Bcl2 resulting in the enhancement of the anti-apoptotic effect of Bcl2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Bax targets mitochondrial membranes, inducing mitochondrial damage and cell death in a caspase-independent manner. Bad plays a critical role in the Bax-mediated apoptosis pathway by dimerizing with BclxL, causing the displacment of Bax. The displacement of Bax allows apoptosis to proceed. BclxS, a shorter version of BclxL (lacking amino acids 126-188), apparently utilizes a different pathway than Bax to induce cell death. Some research suggests that BclxS uses a novel mechanism for regulating caspase or it may use an alternate cell death effector pathway.

Database

Research Area

DATA IMAGES
Bax-antibody-2D3_WB_GTX16119-1_18121409_882.jpg

GTX16119 WB Image

MCF-7 activated with 1 μM Dexamethasone was separated on SDS-PAGE and probed with Monoclonal PA to BAX (MO) CL:2D2 (GTX16119). The antibody was developed with with Alkaline Phosphatase APA Mouse Fab ads HIgG conjugate and an NBT/BCIP substrate. Antibody dilution 5 μg/mL.

Package List Price ($)
$ 319