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Bax antibody [BAX/962]

Anti-Bax antibody [BAX/962] used in IHC (Paraffin sections) (IHC-P). GTX34421
Anti-Bax antibody [BAX/962] used in IHC (Paraffin sections) (IHC-P). GTX34421

Cat No. GTX34421

Host Mouse
Clonality Monoclonal
Clone Name BAX/962
Isotype IgG1
Application WB, ICC/IF, IHC-P
Reactivity Human, Monkey
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.5-1ug/ml
ICC/IF 1-2ug/ml
IHC-P 0.5-1ug/ml for 30 minutes at RT

Note :

IHC-P
Boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Specificity/Sensitivity

Recognizes a protein of 21kDa, identified as the Bax protein. This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein. Bcl-2 blocks cell death following a variety of stimuli. Bax has extensive amino acid homology with Bcl-2 and it homodimerizes and forms heterodimers with Bcl-2. Overexpression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3 dependent cell line, and Bax also counters the death repressor activity of Bcl-2.
PROPERTIES

Form

Liquid

Buffer

Prepared in 10mM PBS with 0.05% BSA and 0.05% azide.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Recombinant full-length human BAX protein.

Purification

Ab purified from Bioreactor Concentrate by Protein A/G

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated X, Apoptosis Regulator,Bcl2L4,Bax

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES
Anti-Bax antibody [BAX/962] used in IHC (Paraffin sections) (IHC-P). GTX34421

GTX34421 IHC-P Image

Formalin-fixed, paraffin-embedded human Melanoma stained with Bax Monoclonal Antibody (BAX/962).

Anti-Bax antibody [BAX/962] used in IHC (Paraffin sections) (IHC-P). GTX34421

GTX34421 IHC-P Image

Formalin-fixed, paraffin-embedded Hodgkin's Lymphoma stained with Bax Monoclonal Antibody (BAX/962).

Package List Price ($)
$ 329