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Bax antibody [HL236]

Recombinant antibody KO/KD Orthogonal
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715
Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715
Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

Cat. No. GTX635715

Reference 3

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL236

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all Bax products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Predict Reactivity

Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_2888571

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated X, apoptosis regulator , BCL2L4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

KO/KD

Wild-type (WT) and Bax knockout (KO) Expi293 cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

KO/KD

Wild-type (WT) and NME4 knockout (KO) HeLa cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Raw264.7 whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded rat tissues.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein by immunohistochemical analysis.
Sample: Paraffin-embedded mouse tissues.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Orthogonal

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bax antibody [HL236] used in IHC (Paraffin sections) (IHC-P). GTX635715

GTX635715 IHC-P Image

Bax antibody [HL236] detects Bax protein at cytoplasm by immunohistochemical analysis.
Sample: Paraffin-embedded mouse kidney.
Bax stained by Bax antibody [HL236] (GTX635715) diluted at 1:50.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-Bax antibody [HL236] used in Western Blot (WB). GTX635715

GTX635715 WB Image

Various whole cell extracts (30 μg) were separated by 15% SDS-PAGE, and the membrane was blotted with Bax antibody [HL236] (GTX635715) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Li Y et al. Transl Lung Cancer Res 2021; 10 (4) : 1841-1856 Let-7b-3p inhibits tumor growth and metastasis by targeting the BRF2-mediated MAPK/ERK pathway in human lung adenocarcinoma.
Application : WB
Reactivity : Human
Qi Y et al. Cancer Cell Int 2021; 21 (1) : 74
Application : WB
Reactivity : Human
Huang YY et al. BMC Vet Res 2020; 16 (1) : 193 Inhibiting autophagy potentiates the antitumor efficacy of Euphorbia royleana for canine mammary gland tumors.
Application : WB
Submit a Reference

REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations