GeneTex
  • Country / Location Selection

United States (US)

Bax antibody [N1N2], N-term

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683
Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683
Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683
Anti-Bax antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109683
Anti-Bax antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109683
Anti-Bax antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109683
Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683
Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

Cat No. GTX109683

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr, IP

Reactivity

Human, Mouse, Rat
Package
100 μl ($379),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
IP 1:100-1:500
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Positive Control

Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2

Predict Reactivity

Bovine, Cat, Dog, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS pH7, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1949720

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

BCL2 associated X, apoptosis regulator , BCL2L4

Cellular Localization

Isoform Alpha: Mitochondrion membrane , Cytoplasm , Isoform Beta: Cytoplasm , Isoform Gamma: Cytoplasm , Isoform Delta: Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

GTX109683 WB Image

Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

GTX109683 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

GTX109683 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-Bax antibody [N1N2], N-term used in Immunoprecipitation (IP). GTX109683

GTX109683 IP Image

Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term (GTX109683)
15 % SDS-PAGE
The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]

Anti-Bax antibody [N1N2], N-term used in IHC (Paraffin sections) (IHC-P). GTX109683

GTX109683 IHC-P Image

Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-Bax antibody [N1N2], N-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109683

GTX109683 ICC/IF Image

Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bax protein stained by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:400.
Blue: Hoechst 33342 staining.

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

GTX109683 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.

Anti-Bax antibody [N1N2], N-term used in Western Blot (WB). GTX109683

GTX109683 WB Image

Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

REVIEW

Bax antibody [N1N2], N-term Cat No. GTX109683

Rating ( Average 5 based on 16 users reviews)
Application
Western Blot(WB) ( Average 5 based on 15 users reviews)
IHC (Frozen sections)(IHC-Fr) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 10-Sep-2021
Score :
Application Tested : IHC-Fr
Sample Species : Rat
Sample : hippocampus
Blocking : 1% BSA in PBST, RT, 1Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : 1% Triton and Citrate Buffer
Primary Antibody : 1:100, 4°C, > 12Hr
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : MDA-MB-231 cells
Amount used : 35
Blocking : 5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody :
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126
Date : Anonymous submitted on 10-Aug-2021
Score :
Application Tested : WB
Sample Species : Rat
Figure Note : The data was peer reviewed and published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
Date : Anonymous submitted on 05-Aug-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 12Hr
Figure Note : 0-30 μM cisplatin treatment for 24hr.
Date : Anonymous submitted on 03-Jun-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : Human brain tumor lysate
Amount used : 30 μg
Blocking : 5% milk in TBST, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Secondary Antibody : GTX300119, 1:2000, 25°C, 1Hr
Date : Anonymous submitted on 30-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Sample : HepG2 cells
Amount used : 20 μg
Blocking : 5% non-fat milk in TTBS, 22°C, 1Hr
Primary Antibody : 1:2000, 4°C, 18Hr
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal PLoS One in 2016. PMID: 26859149
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Molecules in 2017. PMID: 28531143
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2017. PMID: 28467781
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26716417
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26716417
Date : Anonymous submitted on 18-Mar-2021
Score :
Application Tested : WB
Sample Species : Hu
Figure Note : The data was peer reviewed and published in the journal Oncotarget in 2016. PMID: 26716417
Date : Anonymous submitted on 26-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MG63 cells
Amount used : 30 μg
Blocking : 5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody : 1:1000, 4°C, 16Hr
Lane Description : Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date : Anonymous submitted on 15-Jan-2013
Score :
Application Tested : WB
Sample Species : Hu
Sample : U2OS cells
Amount used : 30μg
Blocking : 5% milk, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 16Hr
Application Reference
SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 379
$ 169