APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Recommended Dilution |
1:500-1:3000 |
1:100-1:1000 |
1:100-1:1000 |
Assay dependent |
1:100-1:500 |
Not tested in other applications.
Calculated MW
Positive Control
Neuro2A , NIH-3T3 , Raw264.7 , C2C12 , PC-12 , Rat-2
Predict Reactivity
Bovine, Cat, Dog, Rhesus Monkey(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.27 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bax. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1949720
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
BCL2 associated X, apoptosis regulator , BCL2L4
Cellular Localization
Isoform Alpha: Mitochondrion membrane , Cytoplasm , Isoform Beta: Cytoplasm , Isoform Gamma: Cytoplasm , Isoform Delta: Cytoplasm
Background
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX109683 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109683 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109683 ICC/IF Image
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Bax protein stained by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:400. Blue: Hoechst 33342 staining.
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GTX109683 IHC-P Image
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX109683 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109683 WB Image
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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GTX109683 IP Image
Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments. IP samples: MCF-7 whole cell extract A. Control with 4 μg of preimmune Rabbit IgG B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term (GTX109683) 15 % SDS-PAGE The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500. [EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
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GTX109683 WB Image
Various tissue extracts (50 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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GTX109683 WB Image
The data was published in the journal Stem Cell Res Ther in 2017.PMID: 28482861
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GTX109683 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126
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GTX109683 WB Image
The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557
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GTX109683 WB Image
The data was published in the journal Oncotarget in 2017. PMID: 28467781
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GTX109683 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26716417
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GTX109683 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26716417
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GTX109683 WB Image
The data was published in the journal Oncotarget in 2016. PMID: 26716417
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GTX109683 WB Image
The data was published in the journal PLoS One in 2016. PMID: 26859149
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GTX109683 WB Image
The data was published in the journal Molecules in 2017. PMID: 28531143
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REFERENCE
REVIEW
Bax antibody [N1N2], N-term
Cat. No. GTX109683
Rating
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( Average 5 based on 8 users reviews)
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Application
Western Blot(WB)
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( Average 5 based on 7 users reviews)
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IHC (Frozen sections)(IHC-Fr)
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( Average 5 based on 1 users reviews)
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Date :
Anonymous submitted on 08-Feb-2023
Application Tested :
WB
Sample Species :
Ms
Sample :
Mouse brain tissue lysate
Amount used :
10ug
Blocking :
5% non-fat milk in TBST, 25°C, 0.5Hr
Primary Antibody :
1:500, 16°C, 25Hr
Date :
Anonymous submitted on 10-Sep-2021
Application Tested :
IHC-Fr
Sample Species :
Rat
Sample :
hippocampus
Blocking :
1% BSA in PBST, RT, 1Hr
Fixation :
4% PFA
Permeabilization /Antigen retrieval :
1% Triton and Citrate Buffer
Primary Antibody :
1:100, 4°C, > 12Hr
Date :
Anonymous submitted on 10-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
MDA-MB-231 cells
Amount used :
35
Blocking :
5% milk in TBST buffer, 25°C, 1Hr
Primary Antibody :
Date :
Anonymous submitted on 05-Aug-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 12Hr
Figure Note :
0-30 μM cisplatin treatment for 24hr.
Date :
Anonymous submitted on 03-Jun-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
Human brain tumor lysate
Amount used :
30 μg
Blocking :
5% milk in TBST, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr
Secondary Antibody :
GTX300119, 1:2000, 25°C, 1Hr
Date :
Anonymous submitted on 30-Mar-2021
Application Tested :
WB
Sample Species :
Hu
Sample :
HepG2 cells
Amount used :
20 μg
Blocking :
5% non-fat milk in TTBS, 22°C, 1Hr
Primary Antibody :
1:2000, 4°C, 18Hr
Date :
Anonymous submitted on 26-May-2018
Application Tested :
WB
Sample Species :
Hu
Sample :
MG63 cells
Amount used :
30 μg
Blocking :
5% non-fat dry milk in TBST, 25°C, 1Hr
Primary Antibody :
1:1000, 4°C, 16Hr
Lane Description :
Lane 1: Untreated MG63 whole cell lysate, Lane 2: Treated (IOX-1; 3uM) MG63 whole cell lysate, Lane 3: Treated (IOX-1; 10uM) MG63 whole cell lysateA:Untreated MG63 whole cell lysate
Date :
Anonymous submitted on 15-Jan-2013
Application Tested :
WB
Sample Species :
Hu
Sample :
U2OS cells
Amount used :
30μg
Blocking :
5% milk, 25°C, 1Hr
Primary Antibody :
1:2000, 4°C, 16Hr