Bax antibody [N1N2], N-term
Wild-type (WT) and Bax knockout (KO) 239T cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Bax antibody [N1N2], N-term immunoprecipitates BCL2-associated X protein in IP experiments.
IP samples: MCF-7 whole cell extract
A. Control with 4 μg of preimmune Rabbit IgG
B. Immunoprecipitation of BCL2-associated X protein by 4 μg Bax antibody [N1N2], N-term (GTX109683)
15 % SDS-PAGE
The immunoprecipitated BCL2-associated X protein was detected by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Various whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with Bax antibody [N1N2], N-term (GTX109683) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm on human lung carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded human lung carcinoma.
Bax antibody [N1N2], N-term (GTX109683) diluted at 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
Bax antibody [N1N2], N-term detects Bax protein at cytoplasm, nucleus and mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Bax protein stained by Bax antibody [N1N2], N-term (GTX109683) diluted at 1:400.
Blue: Hoechst 33342 staining.
The data was published in the journal Evid Based Complement Alternat Med in 2012. PMID: 23091557
The data was published in the journal Evid Based Complement Alternat Med in 2013.PMID: 23573126
The data was published in the journal Molecules in 2017. PMID: 28531143
The data was published in the journal PLoS One in 2016. PMID: 26859149
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HostRabbit
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ClonalityPolyclonal
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IsotypeIgG
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ApplicationsWB ICC/IF IHC-P IHC-Fr IP
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ReactivityHuman, Mouse, Rat