*Optimal dilutions/concentrations should be determined by the researcher.
|1-2μg/ml for 30 minutes at RT
Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.
Not tested in other applications.
This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein.
This product does not react with Mouse,Rat.
10mM PBS, 0.05% BSA, 0.05% sodium azide (Please contact us for PBS only format)
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.
Protein A/G purified
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
BAX , BCL2 associated X, apoptosis regulator , BCL2L4 , Bax
The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]
GTX34420 IHC-P Image
IHC-P analysis of human Hodgkin's lymphoma tissue using GTX34420 Bax antibody [SPM336].
GTX34420 ICC/IF Image
ICC/IF analysis of Jurkat cells using GTX34420 Bax antibody [SPM336].
Green : Primary antibody
Red : nucleus
GTX34420 FACS Image
FACS analysis of Jurkat cells using GTX34420 Bax antibody [SPM336].
Blue : Primary antibody
Red : Isotype control