Bax antibody [SPM336]

Cat. No. GTX34420

Host	Mouse
Clonality	Monoclonal
Clone Name	SPM336
Isotype	IgG1
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human, Monkey
Package	100 μg ($529)

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Datasheet
Document

View product citations for antibody GTX34420 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-2μg/ml
ICC/IF	1-2μg/ml
IHC-P	1-2μg/ml for 30 minutes at RT
FACS	1-2μg/10⁶ cells
Note : IHC-P Staining of formalin-fixed tissues require heating tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 45 min at 95ºC followed by cooling at RT for 20 minutes.

Not tested in other applications.

Calculated MW

21 kDa. ( Note )

Product Note

This MAb is highly specific to Bax and shows no cross-reaction with Bcl-2 or Bcl-X protein.
We do not recommend use of this product for Mouse,Rat samples.

PROPERTIES

Form

Liquid

Buffer

PBS, 0.05% BSA

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide, aa 3-16 (Cys-GSGEQPRGGGPTSS) of human bax protein.

Purification

Protein A/G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BAX , BCL2 associated X, apoptosis regulator , BCL2L4 , Bax

Cellular Localization

Mitochondrion membrane,Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Gene ID: 581 BAX
UniProt: Q07812 BAX

Research Area

DATA IMAGES

GTX34420 ICC/IF Image

ICC/IF analysis of Jurkat cells using GTX34420 Bax antibody [SPM336].
Green : Primary antibody
Red : nucleus

GTX34420 IHC-P Image

IHC-P analysis of human Hodgkin's lymphoma tissue using GTX34420 Bax antibody [SPM336].

GTX34420 FACS Image

FACS analysis of Jurkat cells using GTX34420 Bax antibody [SPM336].
Blue : Primary antibody
Red : Isotype control

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 529

Qty