GeneTex
United States (US)

Bax antibody

Cat No. GTX16143

Host Sheep
Clonality Polyclonal
Isotype IgG
Application IP
Reactivity Human
APPLICATION

Application Note

IP: Use at an assay dependent dilution. Is unsuitable for WB. Will not detect denatured protein. Optimal dilutions/concentrations should be determined by the end user.

Calculated MW

21 kDa. ( Note )
PROPERTIES

Form

Liquid

Buffer

Preservative: 0.08% Sodium Azide. Constituents: PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide: LTTAPAPPSLPPATPLGPWAFWSRSQWC, corresponding to amino acids 172-199 of Human beta Bax.

Purification

Protein G purified
This antibody is provided as a 0.2 μm sterile filtered solution.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Bcl2 Associated X, Apoptosis Regulator,Bcl2L4,Bax

Cellular Localization

Cytoplasmic

Background

beta Bax is encoded by a 1.5 kb RNA species created by the retention of intron 5 in the Bax mRNA transcript. The exon4/intron5 splice junction is evolutionarily conserved and is found in both the human and mouse Bax coding regions. The 630 bp intron 5 contributes 60 amino acids to the beta Bax protein before a stop codon is encountered during translation. The beta Bax protein is 24 kDa in size and although it contains the BH1, BH2, and BH3 domains identical to Bax, it has a unique carboxyl terminus and does not have a hydrophobic transmembrane domain. beta Bax mRNA has been found in many mouse tissues using RT-PCR. It is found co-expressed with alpha Bax in lung, stomach, kidney, thymus, lymph node, bone marrow, pancreas, and spleen. beta Bax mRNA was very abundant in brain and duodenum with almost no alpha Bax mRNA detected in these tissues.

Database

Research Area

Package List Price ($)
$ 319