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Bax antibody

KO/KD
Anti-Bax antibody used in IHC (Paraffin sections) (IHC-P). GTX32465
Anti-Bax antibody used in Western Blot (WB). GTX32465
Anti-Bax antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32465
Anti-Bax antibody used in Western Blot (WB). GTX32465

Cat. No. GTX32465

Reference 9

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399)
See all Bax products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:100
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BAX (NP_620116.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887658

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated X, apoptosis regulator , BCL2L4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bax antibody used in IHC (Paraffin sections) (IHC-P). GTX32465

GTX32465 IHC-P Image

IHC-P analysis of human esophageal cancer tissue using GTX32465 Bax antibody.
Dilution : 1:100

Anti-Bax antibody used in Western Blot (WB). GTX32465

GTX32465 WB Image

WB analysis of various sample lysates using GTX32465 Bax antibody.
Dilution : 1:500
Loading : 25μg per lane

Anti-Bax antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX32465

GTX32465 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX32465 Bax antibody.
Blue : DAPI
Dilution : 1:100

Anti-Bax antibody used in Western Blot (WB). GTX32465

GTX32465 WB Image

KO/KD

WB analysis of normal (control) and knockout (KO) 293T cell lysate using GTX32465 Bax antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

Application Reference

Abd-Eldayem AM et al. Sci Rep 2024; 14 (1) : 7434 Cyclosporine-induced kidney damage was halted by sitagliptin and hesperidin via increasing Nrf2 and suppressing TNF-α, NF-κB, and Bax.
Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1) : 28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.
Application : WB
Reactivity : Rat
Jianing Luo et al. Neurosci Bull 2021; 37 (2) : 242-254 Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.
Application : WB
Reactivity : Mouse
Wu X et al. Chin Neurosurg J 2020; 6 : 1 SIRT3 protects against early brain injury following subarachnoid hemorrhage via promoting mitochondrial fusion in an AMPK dependent manner.
Wu X et al. Cell Prolif 2020; 53 (2) : e12759 Recombinant adiponectin peptide promotes neuronal survival after intracerebral haemorrhage by suppressing mitochondrial and ATF4-CHOP apoptosis pathways in diabetic mice via Smad3 signalling inhibition.
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REVIEW

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Application Reference
Abd-Eldayem AM et al. Sci Rep 2024; 14 (1):7434 Cyclosporine-induced kidney damage was halted by sitagliptin and hesperidin via increasing Nrf2 and suppressing TNF-α, NF-κB, and Bax.
Wenbiao Chen et al. Cell Commun Signal 2022; 20 (1):28 Ameliorating role of microRNA-378 carried by umbilical cord mesenchymal stem cells-released extracellular vesicles in mesangial proliferative glomerulonephritis.
Application : WB
Reactivity : Rat
Jianing Luo et al. Neurosci Bull 2021; 37 (2):242-254 Antagonism of Protease-Activated Receptor 4 Protects Against Traumatic Brain Injury by Suppressing Neuroinflammation via Inhibition of Tab2/NF-κB Signaling.
Application : WB
Reactivity : Mouse
Wu X et al. Chin Neurosurg J 2020; 6:1 SIRT3 protects against early brain injury following subarachnoid hemorrhage via promoting mitochondrial fusion in an AMPK dependent manner.
Wu X et al. Cell Prolif 2020; 53 (2):e12759 Recombinant adiponectin peptide promotes neuronal survival after intracerebral haemorrhage by suppressing mitochondrial and ATF4-CHOP apoptosis pathways in diabetic mice via Smad3 signalling inhibition.
Wu X et al. Neurosci Bull 2020; (Epub) Acrolein Aggravates Secondary Brain Injury After Intracerebral Hemorrhage Through Drp1-Mediated Mitochondrial Oxidative Damage in Mice.
Application : WB
Reactivity : Mouse
Wu X et al. Transl Stroke Res 2020; (Epub) Recombinant Adiponectin Peptide Ameliorates Brain Injury Following Intracerebral Hemorrhage by Suppressing Astrocyte-Derived Inflammation via the Inhibition of Drp1-Mediated Mitochondrial Fission.
Application : WB
Reactivity : Mouse
Jiao L et al. Cell Death Dis 2019; 10 (12):942 lncRNA-ZFAS1 induces mitochondria-mediated apoptosis by causing cytosolic Ca2+ overload in myocardial infarction mice model.
Eid W et al. J Cell Biochem 2017; (Epub) neferine enhances the antitumor effect of *-c in hela cells through the activation of p38-mapk pathway.
Application : WB
Reactivity : Human
SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399