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Bax antibody

Anti-Bax antibody used in IHC (Paraffin sections) (IHC-P). GTX56246
Anti-Bax antibody used in Western Blot (WB). GTX56246

Cat. No. GTX56246

Reference 3

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all Bax products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of Bax. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887944

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

BCL2 associated X, apoptosis regulator , BCL2L4

Cellular Localization

Cytoplasm

Background

The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-Bax antibody used in IHC (Paraffin sections) (IHC-P). GTX56246

GTX56246 IHC-P Image

IHC-P analysis of formalin fixed human lung cancer tissue section using GTX56246 Bax antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-Bax antibody used in Western Blot (WB). GTX56246

GTX56246 WB Image

WB analysis of A2780 (A), MCF7 (B) whole cell lysates using GTX56246 Bax antibody.

REFERENCE

Application Reference

Zhou B et al. Chin Med 2023; 18 (1) : 139 Rosmarinic acid treatment protects against lethal H1N1 virus-mediated inflammation and lung injury by promoting activation of the h-PGDS-PGD(2)-HO-1 signal axis.
Zhao X et al. Cancer Cell Int 2021; 21 (1) : 644 Silencing of the lncRNA H19 enhances sensitivity to X-ray and carbon-ions through the miR-130a-3p /WNK3 signaling axis in NSCLC cells.
Application : WB
Reactivity : Human
Liu Y et al. Technol Cancer Res Treat 2016; 15 (3) : 487-97 Effects of Oxymatrine on the Proliferation and Apoptosis of Human Hepatoma Carcinoma Cells.
Application : IHC-P
Reactivity : Mouse
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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399